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Product Info Summary
| SKU: | M00277 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00277
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody catalog # M00277. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00277)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FOF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Chk2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00277 is reactive to CHEK2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
60915 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00277 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell, 293T cell
IHC: human testis tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded human testis, using Chk2 Antibody.
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Immunofluorescent analysis of Hela cells, using Chk2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CHEK2 (Source: Uniprot.org, NCBI)
Gene Name
CHEK2
Full Name
Serine/threonine-protein kinase Chk2
Weight
60915 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk2;2.7.11.1;CHK2 checkpoint homolog;Cds1 homolog;Hucds1;hCds1;Checkpoint kinase 2;CHEK2;CDS1, CHK2, RAD53; CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK2, check out the CHEK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody (M00277)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody
Question
Would anti-Chk2 Rabbit Monoclonal antibody M00277 work for IP with tibial nerve?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
According to the expression profile of tibial nerve, CHEK2 is highly expressed in tibial nerve. So, it is likely that anti-Chk2 Rabbit Monoclonal antibody M00277 will work for IP with tibial nerve.
Boster Scientific Support
Answered: 2020-04-07
Question
I see that the anti-Chk2 Rabbit Monoclonal antibody M00277 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-13
Question
I would like using your anti-Chk2 Rabbit Monoclonal antibody for li-fraumeni syndrome 2 (lfs2) studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We appreciate your inquiry. This M00277 anti-Chk2 Rabbit Monoclonal antibody is validated on hela cell lysate, 293t cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-12
Question
Is a blocking peptide available for product anti-Chk2 Rabbit Monoclonal antibody (M00277)?
J. Patel
Verified customer
Asked: 2019-08-29
Answer
We do provide the blocking peptide for product anti-Chk2 Rabbit Monoclonal antibody (M00277). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-Chk2 Rabbit Monoclonal antibody for IP for human tibial nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tibial nerve identification?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
It shows on the product datasheet, M00277 anti-Chk2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
I have a question about product M00277, anti-Chk2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00277 anti-Chk2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
Would M00277 anti-Chk2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can see on the product datasheet, M00277 anti-Chk2 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-Chk2 Rabbit Monoclonal antibody M00277 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-Chk2 Rabbit Monoclonal antibody (M00277) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Do you have a BSA free version of anti-Chk2 Rabbit Monoclonal antibody M00277 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Chk2 Rabbit Monoclonal antibody M00277 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Our lab used your anti-Chk2 Rabbit Monoclonal antibody for IP on colon carcinoma a few years ago. I am using human, and We want to use the antibody for WB next. I am interested in examining colon carcinoma as well as tibial nerve in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
I looked at the website and datasheets of our anti-Chk2 Rabbit Monoclonal antibody and I see that M00277 has been validated on human in both IP and WB. Thus M00277 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-16
Question
Our lab were satisfied with the WB result of your anti-Chk2 Rabbit Monoclonal antibody. However we have observed positive staining in muscle isoform 4: nucleus. using this antibody. Is that expected? Could you tell me where is CHEK2 supposed to be expressed?
H. Wu
Verified customer
Asked: 2018-09-14
Answer
From literature, muscle does express CHEK2. Generally CHEK2 expresses in isoform 2: nucleus. note=isoform 10 is, isoform 4: nucleus., isoform 7: nucleus., isoform 9: nucleus., isoform 12: nucleus., nucleus, pml body. nucleus, nucleoplasm. Regarding which tissues have CHEK2 expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 15361853
Muscle, Pubmed ID: 15489334
T-cell, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-14
Question
We are interested in to test anti-Chk2 Rabbit Monoclonal antibody M00277 on human tibial nerve for research purposes, then I may be interested in using anti-Chk2 Rabbit Monoclonal antibody M00277 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
The products we sell, including anti-Chk2 Rabbit Monoclonal antibody M00277, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-07
Question
Is this M00277 anti-Chk2 Rabbit Monoclonal antibody reactive to the isotypes of CHEK2?
J. Wu
Verified customer
Asked: 2015-12-31
Answer
The immunogen of M00277 anti-Chk2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Chk2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibial nerve using anti-Chk2 Rabbit Monoclonal antibody M00277. Let me know if you need anything else.
B. Banerjee
Verified customer
Asked: 2015-05-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-04
Question
Please see the WB image, lot number and protocol we used for tibial nerve using anti-Chk2 Rabbit Monoclonal antibody M00277. Please let me know if you require anything else.
C. Thomas
Verified customer
Asked: 2015-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-09
Question
We have seen staining in human colon carcinoma. Do you have any suggestions? Is anti-Chk2 Rabbit Monoclonal antibody supposed to stain colon carcinoma positively?
A. Zhao
Verified customer
Asked: 2013-12-13
Answer
Based on literature colon carcinoma does express CHEK2. Based on Uniprot.org, CHEK2 is expressed in tibial nerve, mammary gland, colon carcinoma, t-cell, muscle, among other tissues. Regarding which tissues have CHEK2 expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 15361853
Muscle, Pubmed ID: 15489334
T-cell, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-12-13
