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Product Info Summary
| SKU: | P00794 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Cdk9 (T186) Antibody
SKU/Catalog Number
P00794
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Cdk9 (T186) Antibody catalog # P00794. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cdk9 (T186) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00794)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00794 is reactive to CDK9 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
42778 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00794 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using Phospho-Cdk9 (T186) polyclonal antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK9 (Phospho-Thr186) antibody
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Western blot analysis of lysates from NIH/3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDK9 (Source: Uniprot.org, NCBI)
Gene Name
CDK9
Full Name
Cyclin-dependent kinase 9
Weight
42778 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 9;2.7.11.22;2.7.11.23;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit;CDK9;CDC2L4, TAK; CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK9, check out the CDK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cdk9 (T186) Antibody (P00794)
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No publications found for P00794
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-Cdk9 (T186) Antibody
Question
Is there a BSA free version of anti-Phospho-Cdk9 (T186) antibody P00794 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Cdk9 (T186) antibody P00794 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
I was wanting to use your anti-Phospho-Cdk9 (T186) antibody for WB for mouse right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right ovary identification?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
As indicated on the product datasheet, P00794 anti-Phospho-Cdk9 (T186) antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
We are interested in using your anti-Phospho-Cdk9 (T186) antibody for negative regulation of mrna polyadenylation studies. Has this antibody been tested with western blotting on 3t3 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
I appreciate your inquiry. This P00794 anti-Phospho-Cdk9 (T186) antibody is validated on 3t3 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-23
Question
Is this P00794 anti-Phospho-Cdk9 (T186) antibody reactive to the isotypes of CDK9?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The immunogen of P00794 anti-Phospho-Cdk9 (T186) antibody is Synthesized peptide derived from human Cdk9 around the phosphorylation site of T186. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-23
Question
Here is the WB image, lot number and protocol we used for right ovary using anti-Phospho-Cdk9 (T186) antibody P00794. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
Will P00794 anti-Phospho-Cdk9 (T186) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Dhar
Verified customer
Asked: 2019-08-19
Answer
You can see on the product datasheet, P00794 anti-Phospho-Cdk9 (T186) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
We have been able to see staining in mouse right ovary. Are there any suggestions? Is anti-Phospho-Cdk9 (T186) antibody supposed to stain right ovary positively?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
According to literature right ovary does express CDK9. According to Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-06
Question
I see that the anti-Phospho-Cdk9 (T186) antibody P00794 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-23
Question
Is a blocking peptide available for product anti-Phospho-Cdk9 (T186) antibody (P00794)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-Phospho-Cdk9 (T186) antibody (P00794). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-Phospho-Cdk9 (T186) antibody P00794 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-12
Answer
The anti-Phospho-Cdk9 (T186) antibody (P00794) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-12
Question
Can you help my question with product P00794, anti-Phospho-Cdk9 (T186) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Banerjee
Verified customer
Asked: 2018-06-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00794 anti-Phospho-Cdk9 (T186) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-11
Question
My boss were satisfied with the WB result of your anti-Phospho-Cdk9 (T186) antibody. However we have seen positive staining in liver nucleus. cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Based on literature, liver does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-12-28
Question
Would anti-Phospho-Cdk9 (T186) antibody P00794 work for WB with right ovary?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to the expression profile of right ovary, CDK9 is highly expressed in right ovary. So, it is likely that anti-Phospho-Cdk9 (T186) antibody P00794 will work for WB with right ovary.
Boster Scientific Support
Answered: 2017-11-20
Question
you antibody to test anti-Phospho-Cdk9 (T186) antibody P00794 on mouse right ovary for research purposes, then I may be interested in using anti-Phospho-Cdk9 (T186) antibody P00794 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Edwards
Verified customer
Asked: 2017-03-29
Answer
The products we sell, including anti-Phospho-Cdk9 (T186) antibody P00794, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Phospho-Cdk9 (T186) antibody P00794. Let me know if you need anything else.
A. Carter
Verified customer
Asked: 2014-02-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-24
