Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M00794 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CDK9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00794
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK9 Rabbit Monoclonal Antibody catalog # M00794. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00794)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GIH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00794 is reactive to CDK9 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
42778 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00794 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
Positive Control
WB: HeLa cell
IHC: human cervix cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Western blot analysis of CDK9 expression in HeLa cell lysate.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human cervix cancer, using CDK9 Antibody.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDK9 (Source: Uniprot.org, NCBI)
Gene Name
CDK9
Full Name
Cyclin-dependent kinase 9
Weight
42778 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 9;2.7.11.22;2.7.11.23;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit;CDK9;CDC2L4, TAK; CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK9, check out the CDK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK9 Rabbit Monoclonal Antibody (M00794)
Hello CJ!
No publications found for M00794
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CDK9 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CDK9 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-CDK9 Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK9 Rabbit Monoclonal antibody M00794. Let me know if you need anything else.
E. Evans
Verified customer
Asked: 2020-04-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-CDK9 Rabbit Monoclonal antibody (M00794)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-CDK9 Rabbit Monoclonal antibody (M00794). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
Will M00794 anti-CDK9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can see on the product datasheet, M00794 anti-CDK9 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-03
Question
Would anti-CDK9 Rabbit Monoclonal antibody M00794 work on bovine IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not tested anti-CDK9 Rabbit Monoclonal antibody M00794 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CDK9 Rabbit Monoclonal antibody M00794 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Do you have a BSA free version of anti-CDK9 Rabbit Monoclonal antibody M00794 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CDK9 Rabbit Monoclonal antibody M00794 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
We need using your anti-CDK9 Rabbit Monoclonal antibody for transcription initiation from rna polymerase ii promoter studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate your inquiry. This M00794 anti-CDK9 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-CDK9 Rabbit Monoclonal antibody M00794 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
The anti-CDK9 Rabbit Monoclonal antibody (M00794) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-29
Question
We have seen staining in human liver. What should we do? Is anti-CDK9 Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
From what I have seen in literature liver does express CDK9. From what I have seen in Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-12-03
Question
I see that the anti-CDK9 Rabbit Monoclonal antibody M00794 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-05
Question
Is this M00794 anti-CDK9 Rabbit Monoclonal antibody reactive to the isotypes of CDK9?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
The immunogen of M00794 anti-CDK9 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK9 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-24
Question
I was wanting to use your anti-CDK9 Rabbit Monoclonal antibody for IF for human right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right ovary identification?
H. Parker
Verified customer
Asked: 2017-10-11
Answer
It shows on the product datasheet, M00794 anti-CDK9 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-11
Question
My team were satisfied with the WB result of your anti-CDK9 Rabbit Monoclonal antibody. However we have seen positive staining in cervix nucleus. cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
From what I have seen in literature, cervix does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-10-04
Question
We are interested in to test anti-CDK9 Rabbit Monoclonal antibody M00794 on human right ovary for research purposes, then I may be interested in using anti-CDK9 Rabbit Monoclonal antibody M00794 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
The products we sell, including anti-CDK9 Rabbit Monoclonal antibody M00794, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-25
Question
Would anti-CDK9 Rabbit Monoclonal antibody M00794 work for IF with right ovary?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
According to the expression profile of right ovary, CDK9 is highly expressed in right ovary. So, it is likely that anti-CDK9 Rabbit Monoclonal antibody M00794 will work for IF with right ovary.
Boster Scientific Support
Answered: 2017-07-03
Question
Can you help my question with product M00794, anti-CDK9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00794 anti-CDK9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-16
Question
We have tried in the past anti-CDK9 Rabbit Monoclonal antibody for IP on cervix in the past. I am using human, and We are going to use the antibody for IF next. I am interested in examining cervix as well as right ovary in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
L. Parker
Verified customer
Asked: 2016-09-26
Answer
I looked at the website and datasheets of our anti-CDK9 Rabbit Monoclonal antibody and it seems that M00794 has been tested on human in both IP and IF. Thus M00794 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-26
Question
See attached the WB image, lot number and protocol we used for right ovary using anti-CDK9 Rabbit Monoclonal antibody M00794. Please let me know if you require anything else.
M. Parker
Verified customer
Asked: 2015-04-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-28
