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Product Info Summary
| SKU: | A00166T14 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Phospho-Cdk1/2/3 (T14) CDK2 Antibody
SKU/Catalog Number
A00166T14
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Cdk1/2/3 (T14) CDK2 Antibody catalog # A00166T14. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cdk1/2/3 (T14) CDK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00166T14)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00166T14 is reactive to CDK2 in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
33930 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00166T14 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of COS7 cells using Phospho-Cdk1/2/3 (T14) polyclonal antibody diluted at 1:2000
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Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)
Gene Name
CDK2
Full Name
Cyclin-dependent kinase 2
Weight
33930 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 2;2.7.11.22 CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK2, check out the CDK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cdk1/2/3 (T14) CDK2 Antibody (A00166T14)
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