Anti-Phospho-Cdc37 (S13) Antibody

Product Name

Anti-Phospho-Cdc37 (S13) Antibody

View all CDC37 Antibodies

SKU/Catalog Number

A02169S13-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-Cdc37 (S13) Antibody catalog # A02169S13-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Cdc37 (S13) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02169S13-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Cdc37 around the phosphorylation site of S13.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02169S13-1 is reactive to CDC37 in Human, Mouse, Rat

Applications

A02169S13-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44468 MW

Background of CDC37

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.

Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of CDC37 using Anti-Phospho-Cdc37 (S13) Antibody (A02169S13-1).

Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C

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Figure 1. Western blotting validation for Anti-Phospho-Cdc37 (S13) Antibody A02169S13-1

Western Blot (WB) analysis of 3T3 cells using Phospho-Cdc37 (S13) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CDC37 (Source: Uniprot.org, NCBI)

Alternative Names

CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog (S. cerevisiae); CDC37 cell division cycle 37 homolog; CDC37A; cell division cycle 37 homolog (S. cerevisiae); Hsp90 chaperone protein kinase-targeting subunit; hsp90 co-chaperone Cdc37; P50CDC37 CDC37 P50CDC37 cell division cycle 37, HSP90 cochaperone hsp90 co-chaperone Cdc37|CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|cell division cycle 37 homolog|hsp90 chaperone protein kinase-targeting subunit

For more info on CDC37, check out the CDC37 Infographic

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In this infographic, you will see the following information for CDC37: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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