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Product Info Summary
| SKU: | P02421 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho CD3 zeta (Tyr142) CD247 Antibody
SKU/Catalog Number
P02421
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho CD3 zeta (Tyr142) CD247 Antibody (Catalog# P02421). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho CD3 zeta (Tyr142) CD247 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02421)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. Immunogen sequence location: 111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02421 is reactive to CD247 in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
18696 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02421 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells treated with UV 15', using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CD247 (Source: Uniprot.org, NCBI)
Gene Name
CD247
Full Name
T-cell surface glycoprotein CD3 zeta chain
Weight
18696 MW
Superfamily
CD3Z/FCER1G family
Alternative Names
CD247;CD3Z;T3Z;TCRZ;T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD antigen CD247 CD247 CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ CD247 molecule T-cell surface glycoprotein CD3 zeta chain|CD247 , zeta subunit|CD3Z , zeta polypeptide (TiT3 complex)|CD3zeta chain|T-cell receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|TCR zeta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD247, check out the CD247 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD247: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho CD3 zeta (Tyr142) CD247 Antibody (P02421)
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No publications found for P02421
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Phospho CD3 zeta (Tyr142) CD247 Antibody
Question
Is a blocking peptide available for product anti-Phospho CD3 zeta (Tyr142) antibody (P02421)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-Phospho CD3 zeta (Tyr142) antibody (P02421). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospho CD3 zeta (Tyr142) antibody P02421. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this P02421 anti-Phospho CD3 zeta (Tyr142) antibody reactive to the isotypes of CD247?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The immunogen of P02421 anti-Phospho CD3 zeta (Tyr142) antibody is A peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. Immunogen sequence location: 111-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-Phospho CD3 zeta (Tyr142) antibody P02421 work on monkey WB with pancreas?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
Our lab technicians have not validated anti-Phospho CD3 zeta (Tyr142) antibody P02421 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Phospho CD3 zeta (Tyr142) antibody P02421 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
Does anti-Phospho CD3 zeta (Tyr142) antibody P02421 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
According to the expression profile of liver, CD247 is highly expressed in liver. So, it is likely that anti-Phospho CD3 zeta (Tyr142) antibody P02421 will work for WB with liver.
Boster Scientific Support
Answered: 2018-11-27
Question
We are currently using anti-Phospho CD3 zeta (Tyr142) antibody P02421 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
B. Krishna
Verified customer
Asked: 2018-03-30
Answer
The anti-Phospho CD3 zeta (Tyr142) antibody (P02421) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-30
