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Product Info Summary
| SKU: | M02421-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Hu CD3 zeta (pY153) Purified CD247 Monoclonal Antibody
SKU/Catalog Number
M02421-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD3 zeta (pY153) Purified CD247 Monoclonal Antibody (Catalog# M02421-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD3 zeta (pY153) Purified CD247 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02421-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-17
Isotype
Mouse IgG1
Immunogen
A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH. The mouse monoclonal antibody EM-17 recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02421-1 is reactive to CD247 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
18696 MW
Background of CD247
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02421-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 2 - 5 μg/ml; positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 μg/ml; positive control: Jurkat cells treated with pervanadate.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Hu CD3 zeta (pY153) Purified CD247 Monoclonal Antibody M02421-1
Western blotting analysis using monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) reacting with particular phosphorylated human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153Protein Target Info & Infographic
Gene/Protein Information For CD247 (Source: Uniprot.org, NCBI)
Gene Name
CD247
Full Name
T-cell surface glycoprotein CD3 zeta chain
Weight
18696 MW
Superfamily
CD3Z/FCER1G family
Alternative Names
CD3 zeta chain, CD247, T3Z, TCRz, IMD25 CD247 CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ CD247 molecule T-cell surface glycoprotein CD3 zeta chain|CD247 , zeta subunit|CD3Z , zeta polypeptide (TiT3 complex)|CD3zeta chain|T-cell receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|TCR zeta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD247, check out the CD247 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD247: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD3 zeta (pY153) Purified CD247 Monoclonal Antibody (M02421-1)
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