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Product Info Summary
| SKU: | A04355S250 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-20S Proteasome alpha (S250) PSMA3 Antibody
View all Proteasome 20S alpha 3 Antibodies
SKU/Catalog Number
A04355S250
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-20S Proteasome alpha (S250) PSMA3 Antibody catalog # A04355S250. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-20S Proteasome alpha (S250) PSMA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04355S250)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04355S250 is reactive to PSMA3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
28433 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04355S250 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using Phospho-20S Proteasome α3 (S250) polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded Human brain. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using Proteasome alpha3 (Phospho-Ser250) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For PSMA3 (Source: Uniprot.org, NCBI)
Gene Name
PSMA3
Full Name
Proteasome subunit alpha type-3
Weight
28433 MW
Superfamily
peptidase T1A family
Alternative Names
Proteasome subunit alpha type-3;3.4.25.1 PSMA3 HC8, PSC3 proteasome 20S subunit alpha 3 proteasome subunit alpha type-3|macropain subunit C8|multicatalytic endopeptidase complex subunit C8|proteasome (prosome, macropain) subunit, alpha type, 3|proteasome component C8|proteasome subunit C8|proteasome subunit alpha 3|proteasome subunit alpha7|testicular secretory protein Li 43
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMA3, check out the PSMA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-20S Proteasome alpha (S250) PSMA3 Antibody (A04355S250)
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