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Product Info Summary
| SKU: | EK1882 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1882
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxnb2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1882)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: L20-P1201
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1882 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1882 is reactive to Plxnb2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1882 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Plexin B2
Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 15 E3; 15 44.68 cM. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1882-CAP | Anti-Mouse Plxnb2 Pre-coated 96-well strip microplate | 1 |
| EK1882-ST | Mouse Plxnb2 Standard | 2 vials, 50 ng/tube |
| EK1882-DA | Mouse Plxnb2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.126 | 0.183 | 0.235 | 0.344 | 0.536 | 0.917 | 1.513 | 2.030 |
Data Example Images
Click image to see more details
Mouse Plexin B2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Plexin B2, Dilution ratio of 1:10, concentration in serum and plasma is 63-100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 192 | 447 | 31 | 200 | 469 |
| Standard deviation | 1.54 | 10.94 | 28.61 | 1.49 | 14.20 | 32.36 |
| CV (%) | 5.3% | 5.7% | 6.4% | 4.8% | 7.1% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Plexin B2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 30 | 31 | 28 | 29 | 1.11 | 3.8% |
| Sample 2 | 192 | 180 | 170 | 178 | 180 | 7.87 | 4.3% |
| Sample 3 | 447 | 475 | 514 | 455 | 472 | 25.90 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For Plxnb2 (Source: Uniprot.Org, NCBI)
Gene Name
Plxnb2
Full Name
Plexin-B2
Weight
205.127kDa
Superfamily
plexin family
Alternative Names
Plexin-B2; Plxnb2 PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3 plexin B2 plexin-B2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Plxnb2, check out the Plxnb2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Plxnb2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine® (EK1882)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine®
Question
Q: Can Plexin B2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
H. Zhang
Verified customer
Asked: 2019-05-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-03
Question
Q: is it okay to use citrate plasma as samples in Mouse Plexin B2 Picokine® ELISA Kit (Catalog # EK1882)?
D. King
Verified customer
Asked: 2018-10-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Plexin B2 causing disruption of its protein structure. Plexin B2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Plexin B2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-30
Question
Q: how to thaw whole blood sample for Plexin B2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Plexin B2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Plexin B2 using the EK1882 Mouse Plexin B2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-04-06
Question
Q: What is the optimal O.D. value for Plexin B2 ELISA kit? I used your Plexin B2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Plexin B2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Plexin B2 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of Plexin B2 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2018-01-26
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Parker
Verified customer
Asked: 2015-07-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-07-21
