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Product Info Summary
| SKU: | A00427-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EPO Receptor/Epor Antibody Picoband®
View all Erythropoietin R Antibodies
SKU/Catalog Number
A00427-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPO Receptor/Epor Antibody Picoband® catalog # A00427-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPO Receptor/Epor Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00427-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse EPO Receptor recombinant protein (Position: D32-E225).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00427-2 is reactive to Epor in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
55.065kDa
Background of Erythropoietin R
The erythropoietin receptor (EpoR) is a protein that in humans is encoded by the EPOR gene. This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00427-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat lung tissue, human U87-MG whole cell, human A431 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EPO Receptor using anti-EPO Receptor antibody (A00427-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO Receptor antigen affinity purified polyclonal antibody (Catalog # A00427-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO Receptor at approximately 55KD. The expected band size for EPO Receptor is at 55KD.
Click image to see more details
Figure 2. Western blot analysis of EPO Receptor using anti-EPO Receptor antibody (A00427-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87-MG whole cell lysates,
Lane 2: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO Receptor antigen affinity purified polyclonal antibody (Catalog # A00427-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO Receptor at approximately 55KD. The expected band size for EPO Receptor is at 55KD.
Protein Target Info & Infographic
Gene/Protein Information For Epor (Source: Uniprot.org, NCBI)
Gene Name
Epor
Full Name
Erythropoietin receptor
Weight
55.065kDa
Superfamily
type I cytokine receptor family
Alternative Names
Erythropoietin receptor; EPO-R; Epor EPOR EPO-R erythropoietin receptor erythropoietin receptor|truncated erythropoietin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Epor, check out the Epor Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Epor: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPO Receptor/Epor Antibody Picoband® (A00427-2)
Hello CJ!
A00427-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao Y, Mengana Y, Cruz Yr, Mu??oz A, Test?? Is, Garc??a Jd, Wu Y, Rodr??guez Jc, Zhang C. J Histochem Cytochem. 2011 Feb;59(2):214-27. Doi: 10.1369/0022155410390323. Different Expression Patterns Of Ngb And Epor In The Cerebral Cortex And Hippocam...
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Customer Q&As
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4 Customer Q&As for Anti-EPO Receptor/Epor Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroid cell using anti-EPO Receptor/Epor antibody A00427-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-EPO Receptor/Epor antibody A00427-2 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The anti-EPO Receptor/Epor antibody (A00427-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-10
Question
I was wanting to use your anti-EPO Receptor/Epor antibody for ELISA for rat erythroid cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat erythroid cell identification?
L. Kulkarni
Verified customer
Asked: 2018-09-05
Answer
It shows on the product datasheet, A00427-2 anti-EPO Receptor/Epor antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroid cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-05
Question
I see that the anti-EPO Receptor/Epor antibody A00427-2 works with ELISA, what is the protocol used to produce the result images on the product page?
S. Moore
Verified customer
Asked: 2018-08-17
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-17
