Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody

EGLN2/PHD1 antibody

Boster Bio Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody catalog # M03015. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M03015
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody

View all EGLN2/PHD1 Antibodies

SKU/Catalog Number

M03015

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody catalog # M03015. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03015)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABD-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PHD1/prolyl hydroxylase

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03015 is reactive to EGLN2 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

35 kDa

Calculated molecular weight

43650 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03015 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Positive Control

WB: HeLa cell, A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For EGLN2 (Source: Uniprot.org, NCBI)

Gene Name

EGLN2

Full Name

Egl nine homolog 2

Weight

43650 MW

Alternative Names

Egl nine homolog 2;1.14.11.29;Estrogen-induced tag 6;HPH-3;Hypoxia-inducible factor prolyl hydroxylase 1;HIF-PH1;HIF-prolyl hydroxylase 1;HPH-1;Prolyl hydroxylase domain-containing protein 1;PHD1;EGLN2;EIT6; EGLN2 EIT-6, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1 egl-9 family hypoxia inducible factor 2 prolyl hydroxylase EGLN2|HIF-prolyl hydroxylase 1|egl nine homolog 2|estrogen-induced tag 6|hypoxia-inducible factor prolyl hydroxylase 1|prolyl hydroxylase domain-containing protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EGLN2, check out the EGLN2 Infographic

EGLN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EGLN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody

Question

My question regards to test anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 on mouse fetal brain for research purposes, then I may be interested in using anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The products we sell, including anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-23

Question

Will M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

It shows on the product datasheet, M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-21

Question

We are currently using anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

G. Krishna

Verified customer

Asked: 2018-11-19

Answer

The anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-19

Question

My question regarding product M03015, anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Li

Verified customer

Asked: 2018-03-08

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-03-08

Question

I see that the anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 works with IF, what is the protocol used to produce the result images on the product page?

L. Wu

Verified customer

Asked: 2017-12-26

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-26

Question

Is a blocking peptide available for product anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015)?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

We do provide the blocking peptide for product anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-02

Order DetailsPrice
M03015

100μL

$370
M03015-30ul

30μL sample

$99
M03015-carrier-free-1ml

Carrier Free

$2590

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M03015
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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