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Product Info Summary
| SKU: | M01018-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PGP9.5 Rabbit Monoclonal Antibody
View all UCH-L1/PGP9.5 Antibodies
SKU/Catalog Number
M01018-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PGP9.5 Rabbit Monoclonal Antibody catalog # M01018-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGP9.5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01018-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOCE-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PGP9.5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01018-1 is reactive to UCHL1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated molecular weight
24824 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01018-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: SH-SY5Y cell
IHC: human brain tissue
Validation Images & Assay Conditions
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Western blot analysis of PGP9.5 expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded human brain, using PGP9.5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For UCHL1 (Source: Uniprot.org, NCBI)
Gene Name
UCHL1
Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Weight
24824 MW
Superfamily
peptidase C12 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase isozyme L1;UCH-L1;3.4.19.12;6.-.-.-;Neuron cytoplasmic protein 9.5;PGP 9.5;PGP9.5;Ubiquitin thioesterase L1;UCHL1; UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UCHL1, check out the UCHL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UCHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGP9.5 Rabbit Monoclonal Antibody (M01018-1)
Hello CJ!
M01018-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Age-associated changes of the intrinsic nervous system in relation with interstitial cells in the pre-weaning goat rumen Yu Liang,1 Imran Tarique,1 Waseem Ail Vistro,1 Yifei Liu,1 Ziyu Wang,2 Abdul haseeb,1 Noor Samad Gandahi,1 Adeela Iqbal,1 Siyi Wang,
Nude mice multi-drug resistance model of orthotopic transplantation of liver neoplasm and Tc-99m MIBI SPECT on p-glycoprotein
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PGP9.5 Rabbit Monoclonal Antibody
Question
Is this M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody reactive to the isotypes of UCHL1?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The immunogen of M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PGP9.5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-20
Question
See attached the WB image, lot number and protocol we used for anterior cingulate cortex using anti-PGP9.5 Rabbit Monoclonal antibody M01018-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
Can you help my question with product M01018-1, anti-PGP9.5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-23
Question
I am interested in to test anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The products we sell, including anti-PGP9.5 Rabbit Monoclonal antibody M01018-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-20
Question
Does M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
You can see on the product datasheet, M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-18
Question
Our team were well pleased with the WB result of your anti-PGP9.5 Rabbit Monoclonal antibody. However we have observed positive staining in anterior cingulate cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is UCHL1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
From literature, anterior cingulate cortex does express UCHL1. Generally UCHL1 expresses in cytoplasm. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-18
Question
Would anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 work for IF with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to the expression profile of anterior cingulate cortex, UCHL1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 will work for IF with anterior cingulate cortex.
Boster Scientific Support
Answered: 2019-07-16
Question
We have seen staining in mouse lung muscle. Any tips? Is anti-PGP9.5 Rabbit Monoclonal antibody supposed to stain lung muscle positively?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
From what I have seen in literature lung muscle does express UCHL1. From what I have seen in Uniprot.org, UCHL1 is expressed in anterior cingulate cortex, lung muscle, brain, cajal-retzius cell fetal brain cortex, among other tissues. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-16
Question
I see that the anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-01
Question
I was wanting to use your anti-PGP9.5 Rabbit Monoclonal antibody for IF for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse anterior cingulate cortex identification?
P. Jones
Verified customer
Asked: 2018-12-24
Answer
It shows on the product datasheet, M01018-1 anti-PGP9.5 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-24
Question
Is there a BSA free version of anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 available?
R. Collins
Verified customer
Asked: 2018-06-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-PGP9.5 Rabbit Monoclonal antibody M01018-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-07
Question
Is a blocking peptide available for product anti-PGP9.5 Rabbit Monoclonal antibody (M01018-1)?
K. Zhao
Verified customer
Asked: 2016-07-14
Answer
We do provide the blocking peptide for product anti-PGP9.5 Rabbit Monoclonal antibody (M01018-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-14
Question
We have tried in the past anti-PGP9.5 Rabbit Monoclonal antibody for IF on brain a few months ago. I am using rat, and I plan to use the antibody for WB next. My question regards examining brain as well as anterior cingulate cortex in our next experiment. Could give a recommendation on which antibody would work the best for WB?
E. Zhang
Verified customer
Asked: 2016-04-18
Answer
I looked at the website and datasheets of our anti-PGP9.5 Rabbit Monoclonal antibody and it appears that M01018-1 has been tested on rat in both IF and WB. Thus M01018-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-04-18
Question
We are currently using anti-PGP9.5 Rabbit Monoclonal antibody M01018-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
C. Huang
Verified customer
Asked: 2014-02-05
Answer
The anti-PGP9.5 Rabbit Monoclonal antibody (M01018-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-05
