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Product Info Summary
| SKU: | A06447 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PFK-B PFKL Antibody
SKU/Catalog Number
A06447
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PFK-B PFKL Antibody catalog # A06447. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFK-B PFKL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06447)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human K6PL. AA range:691-740
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06447 is reactive to PFKL in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
85018 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06447 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of A549 cells using PFK-B Polyclonal antibody.
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Western Blot (WB) analysis of 3T3 cells using PFK-B Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PFKL (Source: Uniprot.org, NCBI)
Gene Name
PFKL
Full Name
ATP-dependent 6-phosphofructokinase, liver type
Weight
85018 MW
Superfamily
phosphofructokinase type A (PFKA) family
Alternative Names
ATP-dependent 6-phosphofructokinase, liver type ;ATP-PFK ;PFK-L;2.7.1.11 ;6-phosphofructokinase type B;Phosphofructo-1-kinase isozyme B;PFK-B;Phosphohexokinase ;PFKL; PFKL ATP-PFK, PFK-B, PFK-L phosphofructokinase, liver type ATP-dependent 6-phosphofructokinase, liver type|6-phosphofructokinase type B|6-phosphofructokinase, liver type|liver-type 1-phosphofructokinase|phosphofructo-1-kinase isozyme B|phosphohexokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKL, check out the PFKL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PFK-B PFKL Antibody (A06447)
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No publications found for A06447
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PFK-B PFKL Antibody
Question
Does anti-PFK-B antibody A06447 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of kidney, PFKL is highly expressed in kidney. So, it is likely that anti-PFK-B antibody A06447 will work for WB with kidney.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-PFK-B antibody A06447 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-PFK-B antibody (A06447) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Is a blocking peptide available for product anti-PFK-B antibody (A06447)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We do provide the blocking peptide for product anti-PFK-B antibody (A06447). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-06
Question
Is this A06447 anti-PFK-B antibody reactive to the isotypes of PFKL?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The immunogen of A06447 anti-PFK-B antibody is Synthesized peptide derived from the C-terminal region of human PFK-B. at AA rangle: 660-740. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-25
Question
I am interested in to test anti-PFK-B antibody A06447 on human kidney for research purposes, then I may be interested in using anti-PFK-B antibody A06447 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The products we sell, including anti-PFK-B antibody A06447, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-12
Question
I was wanting to use your anti-PFK-B antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
It shows on the product datasheet, A06447 anti-PFK-B antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-25
Question
Would A06447 anti-PFK-B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Miller
Verified customer
Asked: 2017-01-26
Answer
As indicated on the product datasheet, A06447 anti-PFK-B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-26
