Product Info Summary
SKU: | A05465-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-HRI EIF2AK1 Antibody
SKU/Catalog Number
A05465-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HRI EIF2AK1 Antibody catalog # A05465-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HRI EIF2AK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05465-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HRI
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05465-1 is reactive to Eif2ak1 in Human, Monkey, Mouse
Applications
A05465-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
71106 MW
Background of Eif2ak1
Isoform GANP:Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells By similarity. Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Isoform MCM3AP:Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.
Nobuo Sakaguchi, J. Immunol., Apr 2005; 174: 4485 - 4494.
Kazuhiko Kuwahara, Blood, Apr 2000; 95: 2321 - 2328.
Y Kono, K Maeda, Genes Cells, Aug 2002; 7: 821 - 834.
Kazuhiko Kuwahara, PNAS, Aug 2001; 98: 10279.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
![a05465 1 anti hri eif2ak1 antibody wb testing 1 a05465 1 anti hri eif2ak1 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05465-1-anti-hri-eif2ak1-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-HRI EIF2AK1 Antibody A05465-1
Western Blot (WB) analysis of specific cells using HRI polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Eif2ak1 (Source: Uniprot.org, NCBI)
Gene Name
Eif2ak1
Full Name
Eukaryotic translation initiation factor 2-alpha kinase 1
Weight
71106 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; eukaryotic translation initiation factor 2-alpha kinase 1; HCR; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; HRIEC 2.7.11.1; KIAA1369heme regulated initiation factor 2 alpha kinase Eif2ak1|H, HCR, Hri|eukaryotic translation initiation factor 2 alpha kinase 1|eukaryotic translation initiation factor 2-alpha kinase 1|heme-controlled repressor|heme-regulated eukaryotic initiation factor eIF-2-alpha kinase|heme-regulated inhibitor|hemin-sensitive initiation factor 2-alpha kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Eif2ak1, check out the Eif2ak1 Infographic
![Eif2ak1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Eif2ak1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HRI EIF2AK1 Antibody (A05465-1)
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