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Product Info Summary
| SKU: | M04504-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PEN2 Rabbit Monoclonal Antibody
View all PSENEN/PEN2 Antibodies
SKU/Catalog Number
M04504-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PEN2 Rabbit Monoclonal Antibody catalog # M04504-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PEN2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04504-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20P65
Isotype
IgG
Immunogen
A synthesized peptide derived from human PEN2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04504-1 is reactive to PSENEN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
12 kDa
Calculated molecular weight
11951 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04504-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PEN2 using anti-PEN2 antibody (M04504-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEN2 antigen affinity purified monoclonal antibody (Catalog # M04504-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEN2 at approximately 12 kDa. The expected band size for PEN2 is at 12 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PSENEN (Source: Uniprot.org, NCBI)
Gene Name
PSENEN
Full Name
Gamma-secretase subunit PEN-2
Weight
11951 MW
Superfamily
PEN-2 family
Alternative Names
Peptidyl-prolyl cis-trans isomerase FKBP1A;PPIase FKBP1A;5.2.1.8;12 kDa FK506-binding protein;12 kDa FKBP;FKBP-12;Calstabin-1;FK506-binding protein 1A;FKBP-1A;Immunophilin FKBP12;Rotamase;FKBP1A;FKBP1, FKBP12; PSENEN ACNINV2, MDS033, MSTP064, PEN-2, PEN2 presenilin enhancer, gamma-secretase subunit gamma-secretase subunit PEN-2|hematopoietic stem/progenitor cells protein MDS033|presenilin enhancer 2 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSENEN, check out the PSENEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSENEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PEN2 Rabbit Monoclonal Antibody (M04504-1)
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