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Product Info Summary
| SKU: | EK0380-HA |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Hamster |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Chinese Hamster IGF-2 ELISA Kit PicoKine®
View all IGF-II/IGF2 ELISA kits
SKU/Catalog Number
EK0380-HA
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Chinese Hamster IGF-2 ELISA Kit PicoKine® (96 Tests). Quantitate Chinese Igf2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Chinese Hamster IGF-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0380-HA)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A25-E91
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0380-HA for more details
Cross-reactivity
There is no detectable cross-reactivity with IGF-1.
Reactive Species
EK0380-HA is reactive to Igf2 in Hamster samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0380-HA is guaranteed for ELISA in Hamster by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-II/IGF2
Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0380-HA-CAP | Anti-Hamster Igf2 Pre-coated 96-well strip microplate | 1 |
| EK0380-HA-ST | Hamster Igf2 Standard | 2 vials, 10 ng/tube |
| EK0380-HA-DA | Hamster Igf2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.049 | 0.112 | 0.196 | 0.309 | 0.593 | 1.04 | 1.668 | 2.171 |
Data Example Images
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Hamster IGF-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Chinese Hamster IGF-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 478 | 1536 | 2743 | 453 | 1737 | 2968 |
| Standard deviation | 20.55 | 87.55 | 126.2 | 30.8 | 140.7 | 222.6 |
| CV (%) | 4.3 | 5.7 | 4.6 | 6.8 | 8.1 | 7.5 |
Protein Target Info & Infographic
Gene/Protein Information For Igf2 (Source: Uniprot.Org, NCBI)
Gene Name
Igf2
Full Name
Insulin-like growth factor II
Weight
20086 MW
Superfamily
insulin family
Alternative Names
Insulin-like growth factor II;IGF-II;Multiplication-stimulating activity;MSA;Multiplication-stimulating polypeptide;Insulin-like growth factor II;Preptin;Igf2;Igf-2; IGF2 C11orf43, GRDF, IGF-II, PP9974, SRS3 insulin like growth factor 2 insulin-like growth factor II|T3M-11-derived growth factor|insulin-like growth factor 2 (somatomedin A)|insulin-like growth factor type 2|preptin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Igf2, check out the Igf2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Chinese Hamster IGF-2 ELISA Kit PicoKine® (EK0380-HA)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Chinese Hamster IGF-2 ELISA Kit PicoKine®
Question
Q: Can IGF-II ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Scott
Verified customer
Asked: 2019-11-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-11-11
Question
Q: how to analyze ELISA data? I measured IGF-II level in plasma.
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-19
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
A. Jackson
Verified customer
Asked: 2019-02-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-02-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Garcia
Verified customer
Asked: 2016-10-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-10-05
