Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody

Pyruvate Dehydrogenase E1-alpha subunit antibody

Boster Bio Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody catalog # M01906. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01906
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody

View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies

SKU/Catalog Number

M01906

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody catalog # M01906. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01906)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AODI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PDHA1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01906 is reactive to PDHA1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

58 kDa

Calculated molecular weight

43296 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01906 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PDHA1 (Source: Uniprot.org, NCBI)

Gene Name

PDHA1

Full Name

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Weight

43296 MW

Alternative Names

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;1.2.4.1;PDHE1-A type I;PDHA1;PHE1A; PDHA1 PDHA, PDHAD, PDHCE1A, PHE1A pyruvate dehydrogenase E1 subunit alpha 1 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase (lipoamide) alpha 1|pyruvate dehydrogenase E1 alpha 1 subunit|pyruvate dehydrogenase alpha 1|pyruvate dehydrogenase complex, E1-alpha polypeptide 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDHA1, check out the PDHA1 Infographic

PDHA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal Antibody

Question

We are currently using anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal antibody M01906 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

The anti-PDHA1/Pdh E1Alpha Rabbit Monoclonal antibody (M01906) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-23

Question

The customer also wanted to know if we have any recommendations for a positive control for M01906 with pig oocyte samples. Best regards,

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

Please see the following publication for reference. https://www.ncbi.nlm.nih.gov/pubmed/?term=pdha1++pig&tdsourcetag=s_pcqq_aiomsg

Boster Scientific Support

Answered: 2019-01-22

Question

I am find an antibody for PDHA1 on your website (M01906 or A01906). My reaction sample from pig tissue and I want to test PDHA1 expression in pig oocyte sample using IHC method. I am not sure this antibody is working in my sample. Do you have some suggestion to choose M01906 or A01906? Could you send to me an antibody sample sack to test antibody available in my pig sample?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

Abways didn't run IHC test with pig samples. Immunogen sequnce for M01906 is PTDHLITAYRAHGF. According to the sequence homologe, M01906 (CY6656) will have a good chance at reacting with pig oocyte samples for IHC.

Boster Scientific Support

Answered: 2018-02-05

Order DetailsPrice
M01906

100μL

$370
M01906-30ul

30μL sample

$99
M01906-carrier-free-1ml

Carrier Free

$2590

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M01906
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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