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Product Info Summary
| SKU: | A00096-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PDGF Receptor beta/PDGFRB Antibody Picoband®
View all PDGF R beta Antibodies
SKU/Catalog Number
A00096-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDGF Receptor beta/PDGFRB Antibody Picoband® catalog # A00096-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGF Receptor beta/PDGFRB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00096-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDGFRB recombinant protein (Position: E911-L992).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00096-1 is reactive to PDGFRB in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
123.968kDa
Background of PDGF R beta
Beta-type platelet-derived growth factor receptor is a protein that in humans is encoded by the PDGFRB gene. The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00096-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human SGC-7901 whole cell, human K562 whole cell, rat testis tissue, mouse testis tissue
IHC: human placenta tissue, rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PDGFRB using anti-PDGFRB antibody (A00096-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SGC-7901 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified polyclonal antibody (Catalog # A00096-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 120KD. The expected band size for PDGFRB is at 124KD.
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Figure 2. IHC analysis of PDGFRB using anti-PDGFRB antibody (A00096-1).
PDGFRB was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PDGFRB Antibody (A00096-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PDGFRB using anti-PDGFRB antibody (A00096-1).
PDGFRB was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PDGFRB Antibody (A00096-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of PDGFRB using anti-PDGFRB antibody (A00096-1).
PDGFRB was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PDGFRB Antibody (A00096-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)
Gene Name
PDGFRB
Full Name
Platelet-derived growth factor receptor beta
Weight
123.968kDa
Superfamily
protein kinase superfamily
Alternative Names
Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR; PDGFR1 PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 -like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRB, check out the PDGFRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDGF Receptor beta/PDGFRB Antibody Picoband® (A00096-1)
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Customer Q&As
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16 Customer Q&As for Anti-PDGF Receptor beta/PDGFRB Antibody Picoband®
Question
Can you help my question with product A00096-1, anti-PDGF Receptor beta/PDGFRB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00096-1 anti-PDGF Receptor beta/PDGFRB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-01
Question
Does A00096-1 anti-PDGF Receptor beta/PDGFRB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
You can see on the product datasheet, A00096-1 anti-PDGF Receptor beta/PDGFRB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-08
Question
Is there a BSA free version of anti-PDGF Receptor beta/PDGFRB antibody A00096-1 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PDGF Receptor beta/PDGFRB antibody A00096-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
We have tried in the past anti-PDGF Receptor beta/PDGFRB antibody for WB on brain last year. I am using rat, and I plan to use the antibody for ELISA next. I am looking for examining brain as well as ectocervix in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
J. Mitchell
Verified customer
Asked: 2019-12-02
Answer
I took a look at the website and datasheets of our anti-PDGF Receptor beta/PDGFRB antibody and it appears that A00096-1 has been tested on rat in both WB and ELISA. Thus A00096-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-02
Question
Is a blocking peptide available for product anti-PDGF Receptor beta/PDGFRB antibody (A00096-1)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We do provide the blocking peptide for product anti-PDGF Receptor beta/PDGFRB antibody (A00096-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-06
Question
Please see the WB image, lot number and protocol we used for brain using anti-PDGF Receptor beta/PDGFRB antibody A00096-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
I was wanting to use to test anti-PDGF Receptor beta/PDGFRB antibody A00096-1 on rat brain for research purposes, then I may be interested in using anti-PDGF Receptor beta/PDGFRB antibody A00096-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-PDGF Receptor beta/PDGFRB antibody A00096-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
Does anti-PDGF Receptor beta/PDGFRB antibody A00096-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of brain, PDGFRB is highly expressed in brain. So, it is likely that anti-PDGF Receptor beta/PDGFRB antibody A00096-1 will work for WB with brain.
Boster Scientific Support
Answered: 2019-06-26
Question
My question regards using your anti-PDGF Receptor beta/PDGFRB antibody for positive regulation of cell proliferation by vegf-activated platelet derived growth factor receptor signaling pathway studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We appreciate your inquiry. This A00096-1 anti-PDGF Receptor beta/PDGFRB antibody is tested on human placenta tissue, hela whole cell lysates, hepg2 whole cell lysates, k562 whole cell lysates, rat kidney tissue, testis tissue, mouse kidney tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-14
Question
I was wanting to use your anti-PDGF Receptor beta/PDGFRB antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
It shows on the product datasheet, A00096-1 anti-PDGF Receptor beta/PDGFRB antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-27
Question
We are currently using anti-PDGF Receptor beta/PDGFRB antibody A00096-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
The anti-PDGF Receptor beta/PDGFRB antibody (A00096-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-19
Question
Our lab were happy with the WB result of your anti-PDGF Receptor beta/PDGFRB antibody. However we have been able to see positive staining in ectocervix cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
Based on literature, ectocervix does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-PDGF Receptor beta/PDGFRB antibody A00096-1. Let me know if you need anything else.
C. Huang
Verified customer
Asked: 2017-10-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-18
Question
Is this A00096-1 anti-PDGF Receptor beta/PDGFRB antibody reactive to the isotypes of PDGFRB?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The immunogen of A00096-1 anti-PDGF Receptor beta/PDGFRB antibody is E. coli-derived human PDGFRB recombinant protein (Position: E911-L992). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-07
Question
We have observed staining in mouse brain. Do you have any suggestions? Is anti-PDGF Receptor beta/PDGFRB antibody supposed to stain brain positively?
B. Singh
Verified customer
Asked: 2016-04-06
Answer
From literature brain does express PDGFRB. From Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-04-06
Question
I see that the anti-PDGF Receptor beta/PDGFRB antibody A00096-1 works with WB, what is the protocol used to produce the result images on the product page?
P. Patel
Verified customer
Asked: 2014-01-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-01-28
