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Product Info Summary
| SKU: | M00096-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal Antibody
View all PDGF R beta Antibodies
SKU/Catalog Number
M00096-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal Antibody catalog # M00096-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00096-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AGG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDGF Receptor beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00096-1 is reactive to PDGFRB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
123968 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00096-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: SH-SY5Y cell
IHC: human uterus tissue
ICC/IF: 3T3 cell
Validation Images & Assay Conditions
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Western blot analysis of PDGF Receptor beta expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded human uterus, using PDGF Receptor beta Antibody.
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Immunofluorescent analysis of 3T3 cells, using PDGF Receptor beta Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)
Gene Name
PDGFRB
Full Name
Platelet-derived growth factor receptor beta
Weight
123968 MW
Superfamily
protein kinase superfamily
Alternative Names
Platelet-derived growth factor receptor beta;PDGF-R-beta;PDGFR-beta;2.7.10.1;Beta platelet-derived growth factor receptor;Beta-type platelet-derived growth factor receptor;CD140 antigen-like family member B;Platelet-derived growth factor receptor 1;PDGFR-1;CD140b;PDGFRB;PDGFR, PDGFR1; PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 -like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRB, check out the PDGFRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal Antibody (M00096-1)
Hello CJ!
M00096-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
RNA interference targeting the platelet-derived growth factor receptor ? subunit ameliorates experimental hepatic fibrosis in rats
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal Antibody
Question
Is this M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody reactive to the isotypes of PDGFRB?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The immunogen of M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody is A synthesized peptide derived from human PDGF Receptor beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-27
Question
I was wanting to use using your anti-PDGF Receptor beta Rabbit Monoclonal antibody for metanephric comma-shaped body morphogenesis studies. Has this antibody been tested with western blotting on 3t3 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thanks for your inquiry. This M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody is tested on 3t3 cells. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-23
Question
Will M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
You can see on the product datasheet, M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-19
Question
My team were satisfied with the WB result of your anti-PDGF Receptor beta Rabbit Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to literature, brain does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-24
Question
We have observed staining in human ectocervix. Do you have any suggestions? Is anti-PDGF Receptor beta Rabbit Monoclonal antibody supposed to stain ectocervix positively?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
According to literature ectocervix does express PDGFRB. According to Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use your anti-PDGF Receptor beta Rabbit Monoclonal antibody for IP for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can see on the product datasheet, M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
Does anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 work for IP with brain?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
According to the expression profile of brain, PDGFRB is highly expressed in brain. So, it is likely that anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 will work for IP with brain.
Boster Scientific Support
Answered: 2019-10-14
Question
I see that the anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Our lab used your anti-PDGF Receptor beta Rabbit Monoclonal antibody for IHC on brain in a previous project. I am using human, and We intend to use the antibody for ICC next. I am interested in examining brain as well as ectocervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
I have checked the website and datasheets of our anti-PDGF Receptor beta Rabbit Monoclonal antibody and I see that M00096-1 has been tested on human in both IHC and ICC. Thus M00096-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-10
Question
Is a blocking peptide available for product anti-PDGF Receptor beta Rabbit Monoclonal antibody (M00096-1)?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
We do provide the blocking peptide for product anti-PDGF Receptor beta Rabbit Monoclonal antibody (M00096-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-11
Question
We are currently using anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
The anti-PDGF Receptor beta Rabbit Monoclonal antibody (M00096-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-20
Question
Our lab want to know about to test anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 on rat brain for research purposes, then I may be interested in using anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Jones
Verified customer
Asked: 2017-05-26
Answer
The products we sell, including anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-26
Question
Is there a BSA free version of anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 available?
K. Wu
Verified customer
Asked: 2016-12-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-12
Question
Please see the WB image, lot number and protocol we used for brain using anti-PDGF Receptor beta Rabbit Monoclonal antibody M00096-1. Please let me know if you require anything else.
E. Carter
Verified customer
Asked: 2015-02-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-18
Question
Can you help my question with product M00096-1, anti-PDGF Receptor beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Krishna
Verified customer
Asked: 2013-07-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00096-1 anti-PDGF Receptor beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-18
