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Product Info Summary
| SKU: | M00366 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PDGFR alpha Rabbit Monoclonal Antibody
View all PDGFR alpha Antibodies
SKU/Catalog Number
M00366
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDGFR alpha Rabbit Monoclonal Antibody catalog # M00366. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGFR alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00366)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAOE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDGFR alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00366 is reactive to PDGFRA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
150 kDa
Calculated molecular weight
122670 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00366 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of PDGFR expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PDGFRA (Source: Uniprot.org, NCBI)
Gene Name
PDGFRA
Full Name
Platelet-derived growth factor receptor alpha
Weight
122670 MW
Superfamily
protein kinase superfamily
Alternative Names
Platelet-derived growth factor receptor alpha;PDGF-R-alpha;PDGFR-alpha;2.7.10.1;Alpha platelet-derived growth factor receptor;Alpha-type platelet-derived growth factor receptor;CD140 antigen-like family member A;CD140a antigen;Platelet-derived growth factor alpha receptor;Platelet-derived growth factor receptor 2;PDGFR-2;CD140a;PDGFRA;PDGFR2, RHEPDGFRA; PDGFRA CD140A, PDGFR-2, PDGFR2 platelet derived growth factor receptor alpha platelet-derived growth factor receptor alpha|CD140 -like family member A|CD140a |PDGF-R-alpha|alpha-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 2|platelet-derived growth factor receptor, alpha polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRA, check out the PDGFRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDGFR alpha Rabbit Monoclonal Antibody (M00366)
Hello CJ!
M00366 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births
PDGFR%u03B1 regulated by miR-34a and FoxO1 promotes adipogenesis in porcine intramuscular preadipocytes through Erk signaling pathway
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PDGFR alpha Rabbit Monoclonal Antibody
Question
Does M00366 require dry ice during shipping?
Verified customer
Asked: 2021-10-20
Answer
We ship the Anti-PDGFR Alpha Rabbit Monoclonal Antibody (M00366) with gel ice.
Boster Scientific Support
Answered: 2021-10-21
Question
Our lab were happy with the WB result of your anti-PDGFR alpha Rabbit Monoclonal antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to literature, placenta does express PDGFRA. Generally PDGFRA expresses in cell membrane. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-PDGFR alpha Rabbit Monoclonal antibody M00366 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
J. Parker
Verified customer
Asked: 2017-11-03
Answer
The anti-PDGFR alpha Rabbit Monoclonal antibody (M00366) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-03
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-PDGFR alpha Rabbit Monoclonal antibody supposed to stain blood positively?
J. Jones
Verified customer
Asked: 2015-03-30
Answer
Based on literature blood does express PDGFRA. Based on Uniprot.org, PDGFRA is expressed in endometrium, foreskin, brain, blood, lung trachea, placenta, eosinophil, colon tumor, among other tissues. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-03-30
