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Product Info Summary
| SKU: | M04772 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PCK2 Monoclonal Antibody
SKU/Catalog Number
M04772
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PCK2 Monoclonal Antibody catalog # M04772. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04772)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADFB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PCK2 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04772 is reactive to PCK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
42875 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04772 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HepG2 cell, Mouse brain, Rat spleen
Validation Images & Assay Conditions
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Western blot analysis of PCK2 expression in (1) HepG2 cell lysate; (2) Mouse brain lysate; (3) Rat spleen lysate.
Protein Target Info & Infographic
Gene/Protein Information For PCK2 (Source: Uniprot.org, NCBI)
Gene Name
PCK2
Full Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Weight
42875 MW
Superfamily
phosphoenolpyruvate carboxykinase [GTP] family
Alternative Names
Alpha-sarcoglycan;Alpha-SG;50 kDa dystrophin-associated glycoprotein;50DAG;Adhalin;Dystroglycan-2;SGCA;ADL, DAG2; PCK2 PEPCK, PEPCK-M, PEPCK2 phosphoenolpyruvate carboxykinase 2, mitochondrial phosphoenolpyruvate carboxykinase [GTP], mitochondrial|PEP carboxykinase|epididymis secretory sperm binding protein|phosphopyruvate carboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCK2, check out the PCK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCK2 Monoclonal Antibody (M04772)
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No publications found for M04772
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Customer Q&As
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7 Customer Q&As for Anti-PCK2 Monoclonal Antibody
Question
Can you help my question with product M04772, anti-PCK2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04772 anti-PCK2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
I was wanting to use your anti-PCK2 Monoclonal antibody for IF for mouse neuroblastoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse neuroblastoma identification?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
It shows on the product datasheet, M04772 anti-PCK2 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse neuroblastoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
Please see the WB image, lot number and protocol we used for neuroblastoma using anti-PCK2 Monoclonal antibody M04772. Please let me know if you require anything else.
J. Yang
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-PCK2 Monoclonal antibody M04772 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
N. Jones
Verified customer
Asked: 2018-10-23
Answer
The anti-PCK2 Monoclonal antibody (M04772) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
Is there a BSA free version of anti-PCK2 Monoclonal antibody M04772 available?
B. Singh
Verified customer
Asked: 2018-07-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PCK2 Monoclonal antibody M04772 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neuroblastoma using anti-PCK2 Monoclonal antibody M04772. Let me know if you need anything else.
R. Lewis
Verified customer
Asked: 2016-05-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-05
Question
Is a blocking peptide available for product anti-PCK2 Monoclonal antibody (M04772)?
O. Zhao
Verified customer
Asked: 2014-12-19
Answer
We do provide the blocking peptide for product anti-PCK2 Monoclonal antibody (M04772). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-19
