Anti-PCK2 Antibody

Product Name

Anti-PCK2 Antibody

View all PCK2 Antibodies

SKU/Catalog Number

A04772-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-PCK2 Antibody catalog # A04772-2. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04772-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human UGDH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04772-2 is reactive to PCK2 in Human, Mouse, Rat

Applications

A04772-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

70699 MW

Background of PCK2

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Spicer A.P., J. Biol. Chem. 273:25117-25124(1998).
Peng H.L., Proc. Natl. Sci. Counc. Repub. China, B, Life Sci. 22:166-172(1998).
Chang M.L., Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of PCK2 using Anti-PCK2 Antibody (A04772-2).

ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-PCK2 Antibody A04772-2

Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For PCK2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 4.1.1.32; PE; PEPCK; PEPCK2PEP carboxykinase; PEPCK-M; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase 2 (mitochondrial); Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase PCK2 PEPCK, PEPCK-M, PEPCK2 phosphoenolpyruvate carboxykinase 2, mitochondrial phosphoenolpyruvate carboxykinase [GTP], mitochondrial|PEP carboxykinase|epididymis secretory sperm binding protein|phosphopyruvate carboxylase

For more info on PCK2, check out the PCK2 Infographic

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In this infographic, you will see the following information for PCK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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