Product Info Summary
SKU: | A04772-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PCK2 Antibody
SKU/Catalog Number
A04772-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PCK2 Antibody catalog # A04772-2. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04772-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04772-2 is reactive to PCK2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
70699 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04772-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of PCK2 using Anti-PCK2 Antibody (A04772-2).
ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-PCK2 Antibody A04772-2
Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PCK2 (Source: Uniprot.org, NCBI)
Gene Name
PCK2
Full Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Weight
70699 MW
Superfamily
phosphoenolpyruvate carboxykinase [GTP] family
Alternative Names
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial;PEPCK-M;4.1.1.32;PCK2;PEPCK2; PCK2 PEPCK, PEPCK-M, PEPCK2 phosphoenolpyruvate carboxykinase 2, mitochondrial phosphoenolpyruvate carboxykinase [GTP], mitochondrial|PEP carboxykinase|epididymis secretory sperm binding protein|phosphopyruvate carboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCK2, check out the PCK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCK2 Antibody (A04772-2)
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