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Product Info Summary
| SKU: | A06921-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PCH2/TRIP13 Antibody Picoband®
View all Thyroid receptor-interacting protein 13 Antibodies
SKU/Catalog Number
A06921-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCH2/TRIP13 Antibody Picoband® catalog # A06921-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PCH2/TRIP13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06921-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PCH2/TRIP13 recombinant protein (Position: M1-Q421).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06921-2 is reactive to TRIP13 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
87148 MW
Background of Thyroid receptor-interacting protein 13
TRIP13 is a mammalian gene that encodes the thyroid receptor-interacting protein 13. This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06921-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PCH2/TRIP13 using anti-PCH2/TRIP13 antibody (A06921-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCH2/TRIP13 antigen affinity purified polyclonal antibody (Catalog # A06921-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCH2/TRIP13 at approximately 49 kDa. The expected band size for PCH2/TRIP13 is at 49 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-PCH2/TRIP13 antibody (A06921-2).
Overlay histogram showing 293T cells stained with A06921-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCH2/TRIP13 Antibody (A06921-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TRIP13 (Source: Uniprot.org, NCBI)
Gene Name
TRIP13
Full Name
Pachytene checkpoint protein 2 homolog
Weight
87148 MW
Superfamily
AAA ATPase family
Alternative Names
Interleukin-34; IL-34; IL34; C16orf77 TRIP13 16E1BP, MVA3, OOMD9 thyroid hormone receptor interactor 13 pachytene checkpoint protein 2 homolog|16E1-BP|HPV16 E1 protein binding protein|SH3TC1/TRIP13 fusion|TR-interacting protein 13|TRIP-13|human papillomavirus type 16 E1 protein-binding protein|thyroid receptor-interacting protein 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIP13, check out the TRIP13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCH2/TRIP13 Antibody Picoband® (A06921-2)
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