Product Info Summary
SKU: | M01130 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PBRM1 Rabbit Monoclonal Antibody
View all BAF180/PB1 Antibodies
SKU/Catalog Number
M01130
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PBRM1 Rabbit Monoclonal Antibody catalog # M01130. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBRM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01130)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFHG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PBRM1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01130 is reactive to PBRM1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
27774 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01130 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:100
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PBRM1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PBRM1 (Source: Uniprot.org, NCBI)
Gene Name
PBRM1
Full Name
Protein polybromo-1
Weight
27774 MW
Alternative Names
14-3-3 protein sigma;Epithelial cell marker protein 1;Stratifin;SFN;HME1; PBRM1 BAF180, PB1 polybromo 1 protein polybromo-1|BRG1-associated factor 180|polybromo-1D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PBRM1, check out the PBRM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PBRM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PBRM1 Rabbit Monoclonal Antibody (M01130)
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No publications found for M01130
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Customer Q&As
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6 Customer Q&As for Anti-PBRM1 Rabbit Monoclonal Antibody
Question
Does anti-PBRM1 Monoclonal antibody M01130 work for Flow Cytometry with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PBRM1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PBRM1 Monoclonal antibody M01130 will work for Flow Cytometry with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-02-17
Question
What sample was used in your WB analysis of M01130?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-PBRM1 Rabbit Monoclonal Antibody (M01130), western blot analysis of PBRM1 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
I am interested in to test anti-PBRM1 Monoclonal antibody M01130 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-PBRM1 Monoclonal antibody M01130 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-PBRM1 Monoclonal antibody M01130, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
Is there a BSA free version of anti-PBRM1 Monoclonal antibody M01130 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PBRM1 Monoclonal antibody M01130 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
Is a blocking peptide available for product anti-PBRM1 Monoclonal antibody (M01130)?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
We do provide the blocking peptide for product anti-PBRM1 Monoclonal antibody (M01130). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-06
Question
We are currently using anti-PBRM1 Monoclonal antibody M01130 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
The anti-PBRM1 Monoclonal antibody (M01130) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-03