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Product Info Summary
| SKU: | PA1713 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PBP/PEBP1 Antibody Picoband®
SKU/Catalog Number
PA1713
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PBP/PEBP1 Antibody catalog # PA1713. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBP/PEBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1713)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1713 is reactive to Pebp1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
20830 MW
Background of RKIP/PBP
PEBP1 (Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al (2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1713 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat brain tissue, rat liver tissue, mouse brian tissue, mouse liver tissue
IHC: Rat Kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PEBP1 using anti-PEBP1 antibody (PA1713).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse brian tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEBP1 antigen affinity purified polyclonal antibody (Catalog # PA1713) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEBP1 at approximately 21 kDa. The expected band size for PEBP1 is at 21 kDa.
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Figure 2. IHC analysis of PEBP1 using anti-PEBP1 antibody (PA1713).
PEBP1 was detected in a paraffin-embedded section of Rat Kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PEBP1 Antibody (PA1713) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Pebp1 (Source: Uniprot.org, NCBI)
Gene Name
Pebp1
Full Name
Phosphatidylethanolamine-binding protein 1
Weight
20830 MW
Superfamily
phosphatidylethanolamine-binding protein family
Alternative Names
Phosphatidylethanolamine-binding protein 1;PEBP-1;HCNPpp;Hippocampal cholinergic neurostimulating peptide;HCNP;Pebp1;Pbp, Pebp; PEBP1 HCNP, HCNPpp, HEL-210, HEL-S-34, HEL-S-96, PBP, PEBP, PEBP-1, RKIP phosphatidylethanolamine binding protein 1 phosphatidylethanolamine-binding protein 1|Raf kinase inhibitory protein|epididymis luminal protein 210|epididymis secretory protein Li 34|epididymis secretory protein Li 96|hippocampal cholinergic neurostimulating peptide|neuropolypeptide h3|prostatic binding protein|raf kinase inhibitor protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pebp1, check out the Pebp1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pebp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PBP/PEBP1 Antibody Picoband® (PA1713)
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No publications found for PA1713
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Customer Q&As
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4 Customer Q&As for Anti-PBP/PEBP1 Antibody Picoband®
Question
I was wanting to use your anti-PBP/PEBP1 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
As indicated on the product datasheet, PA1713 anti-PBP/PEBP1 antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
Is this PA1713 anti-PBP/PEBP1 antibody reactive to the isotypes of PEBP1?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The immunogen of PA1713 anti-PBP/PEBP1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-23
Question
Is there a BSA free version of anti-PBP/PEBP1 antibody PA1713 available?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PBP/PEBP1 antibody PA1713 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-05
Question
We are currently using anti-PBP/PEBP1 antibody PA1713 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
C. Singh
Verified customer
Asked: 2016-07-19
Answer
The anti-PBP/PEBP1 antibody (PA1713) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-19
