This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Phosphatidylethanolamine-binding protein 1.
Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.
Human | |
---|---|
Gene Name: | PEBP1 |
Uniprot: | P30086 |
Entrez: | 5037 |
Belongs to: |
---|
phosphatidylethanolamine-binding protein family |
HCNP; HCNPpp; hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; PBP; PBPPEBP-1; PEBP1; PEBP-1; PEBPHCNPpp; phosphatidylethanolamine binding protein 1; phosphatidylethanolamine-binding protein 1; prostatic binding protein; Prostatic-binding protein; Raf kinase inhibitory protein; RKIP; RKIPRaf kinase inhibitor protein
Mass (kDA):
21.057 kDA
Human | |
---|---|
Location: | 12q24.23 |
Sequence: | 12; NC_000012.12 (118136124..118145584) |
Cytoplasm.
The PEBP1 marker is a flexible protein that blocks cell proliferation as well as triggering differentiation in human Keratinocytes. Boster Bio developed this marker to aid scientists in determining the types of cells that respond to inhibition by PEBP1. Research can be done with the PEBP1 Recombinant DNA plasmid or shRNA. Here are some examples of the best uses of PEBP1.
Previously, researchers have identified an additional role for PEBP1 in cell expansion. It negatively regulates autophagy in conditions of starvation. This is the reason PEBP1 is a crucial inhibitor of autophagy. Boster Bio's PEBP1 inhibitors slow cell proliferation and decrease autophagy-induced apoptosis. In in vitro, the inhibitors block the autophagy pathway and hinder the growth of cancerous cells.
PEBP1 is a protein which blocks the extracellular signal-regulated Kinase (ERK) pathway. By blocking ERK cell proliferation, it is reduced. To determine if PEBP inhibitors could cause differentiation of human keratinocytes we examined PEBP1 inhibitors. PEBP was not found in the epidermis basal layer, but was present in the spinous and granular layers , as well as in differentiated and undifferentiated human skin melanomas.
PEBP1 is a transmembrane-associated protein that interacts directly with LC3. It interacts with LC3 through an LIR motif in the lab. Boster Bio has demonstrated that these inhibitors block neuropsin and trypsin in animal studies. In addition, they possess the capability to block RAF1 activity and MEK phosphorylation.
In human cell culture, pcDNA3.1-FLAG-RKIP/PEBP1 was transiently transfected into h2299 cells. The proteins were then purified by using an anti-FLAG M2 affinity gel. The bound proteins were removed from the beads by washing them in 2XSDS gel loading buffer. They inhibit cell proliferation in both human as well as animal models.
In vitro experiments also showed that PEBP1S153A is overexpressed, which results in decreased LC3II production. It is possible that the phospho-mimicking mutation aids in the release of LC3 from PEBP1S153A complexes. The mutation could also regulate the interaction of PEBP1 with LC3 and thus regulate cellular responses to starvation.
Boster Bio's PEBP1 inhibitor drugs target cell proliferation by stimulating the activity the PEBP1 gene, which is involved in controlling the growth of cells. These compounds will assist us to identify signalling pathways and interplay between pathways in order to develop molecularly targeted treatments. These drugs are also highly effective against cancers, including colon, breast, lung, and renal cells.
Sanbio offers Boster Bio PEBP1 recombnant plasmid to help scientists in cloning and expressing human PEBP1 in cell cultures. The company's customer support team is ready to assist you with your research projects. Sanbio also provides custom services and BeNeLux delivery. These are just one of the many advantages of working with Boster Bio.
The company was founded in 1993 and has since expanded its product line to include molecular biology products that are PCR-related. Technical support is available all hours of the day and is offered for free. Boster Bio offers technical support for all of its products, as well with recombinant and antibody solutions. The technical support team of the company also provides a wide variety of tools and services that help researchers.
Both the LINC00473-up lentivirus (LINC00473) and PEBP1 recombinant (RNA) (PEBP1) were successfully transfected into hBMSCs. Both the lentiviruses as well the PEBP1-plasmids that were recombinant were very effective in knocking out PEBP1.
Boster Bio's PEBP1 recombidplasmid works extremely well in salvaging hBMSCs. PEBP1 activation activates the Bcl-2/Akt/Bad/Akt pathway. This is crucial in identifying potential targets for LINC00473.
PEBP1 shRNA can be obtained in a variety of forms, such as protein and gene knockdown. This RNA targets the PEBP1 gene and is a valuable tool for research. Boster Bio offers PEBP1 shRNA for many applications. This article will review the most well-known uses for PEBP1 RNA. The most important uses for PEBP1 shRNA are in human research and in the development of cancer-fighting drugs.
All three kinds of sh-PEBP1 have been found to decrease PEBP1 mRNA expression. PEBP1-up significantly raised PEBP1 protein levels. All three kinds of sh-PEBP1 were successful in knocking down PEBP1. The most effective method of controlling PEBP1 mRNA expression was PEBP1-1. The levels of PEBP1 protein were compared with those of sh-Control and control cells.
In the study, PEBP1 shRNA was transfected into hBMSCs that were induced by Dex using the Lipofectamine 2000 system. Three PEBP1 shRNAs were employed each with a distinct target sequence. Genechem Corporation supplied the plasmid for PEBP1 overexpression. After transfection, PEBP1 shRNA knockdown was able restore Akt activity and activate the signaling pathway Akt/Bcl-2.
Boster Bio provides ELISA kits for PEBP1 and a variety of other immunological applications. These kits are able to detect biomarkers related to developmental biology, neuroscience, inflammation, and other immunological applications. The sensitivity of these kits is at the level of a picogram. Boster Bio provides technical support through Sanbio, their BeNeLux distributor. The kits for antibodies are optimized for immunogen design and validated through panels consisting of over 250 tissue samples.
PMID: 8144042 by Hori N., et al. A human cDNA sequence homologue of bovine phosphatidylethanolamine- binding protein.
PMID: 7637590 by Tohdoh N., et al. Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein.