Product Info Summary
SKU: | A01033-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Paxillin/PXN Antibody Picoband®
SKU/Catalog Number
A01033-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Paxillin/PXN Antibody Picoband® catalog # A01033-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Paxillin/PXN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01033-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Paxillin/PXN recombinant protein (Position: A9-K570).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01033-2 is reactive to PXN in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64.505kDa
Background of Paxillin
PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein–protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01033-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human HEK293 whole cell, human HepG2 whole cell, mouse lung tissue
IHC: human laryngeal carcinoma tissue, human hashimoto thyroiditis tissue, human thyroid papillary carcinoma tissue, rat brain tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
![a01033 2 pxn primary antibodies wb testing 1 a01033 2 pxn primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Paxillin/PXN antigen affinity purified polyclonal antibody (Catalog # A01033-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Paxillin/PXN at approximately 65 kDa. The expected band size for Paxillin/PXN is at 65 kDa.
![a01033 2 pxn primary antibodies ihc testing 2 a01033 2 pxn primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2).
Paxillin/PXN was detected in a paraffin-embedded section of human laryngeal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Paxillin/PXN Antibody (A01033-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01033 2 pxn primary antibodies ihc testing 3 a01033 2 pxn primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2).
Paxillin/PXN was detected in a paraffin-embedded section of human hashimoto thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Paxillin/PXN Antibody (A01033-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01033 2 pxn primary antibodies ihc testing 4 a01033 2 pxn primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2).
Paxillin/PXN was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Paxillin/PXN Antibody (A01033-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01033 2 pxn primary antibodies ihc testing 5 a01033 2 pxn primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2).
Paxillin/PXN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Paxillin/PXN Antibody (A01033-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01033 2 pxn primary antibodies fcm testing 6 a01033 2 pxn primary antibodies fcm testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01033-2-pxn-primary-antibodies-fcm-testing-6.jpg)
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Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Paxillin/PXN antibody (A01033-2).
Overlay histogram showing MCF-7 cells stained with A01033-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Paxillin/PXN Antibody (A01033-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PXN (Source: Uniprot.org, NCBI)
Gene Name
PXN
Full Name
Paxillin
Weight
64.505kDa
Superfamily
paxillin family
Alternative Names
Tyrosine-protein kinase Tec; TEC; PSCTK4 PXN paxillin paxillin|testicular tissue protein Li 134
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXN, check out the PXN Infographic
![PXN infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Paxillin/PXN Antibody Picoband® (A01033-2)
Hello CJ!
A01033-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhong S, Luo R, Wang X, Tang L, Wu J, Wang J, Huang R, Sun H, Huang N. Colloids Surf B Biointerfaces. 2014 Apr 1;116:553-60. Doi: 10.1016/J.Colsurfb.2014.01.030. Epub 2014 Jan 30. Effects Of Polydopamine Functionalized Titanium Dioxide Nanotubes O...
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