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Product Info Summary
| SKU: | MA1080 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Chicken, Hamster, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10)
SKU/Catalog Number
MA1080
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) catalog # MA1080. Tested in ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
PXC-10
Isotype
Mouse IgG1
Immunogen
C-terminal part of recombinant chicken paxillin (amino acids 305-559).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1080 is reactive to Pxn in Bovine, Chicken, Hamster, Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64019 MW
Background of Paxillin
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1080 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -
Western blot, 1-2μg/ml, Human, mouse, rat, chicken
Positive Control
WB: human A431 whole cell, human Hela whole cell, human MCF-7 whole cell, human CACO-2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Paxillin using anti-Paxillin antibody (MA1080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Paxillin antigen affinity purified monoclonal antibody (Catalog # MA1080) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Paxillin at approximately 65 kDa. The expected band size for Paxillin is at 65 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Pxn (Source: Uniprot.org, NCBI)
Gene Name
Pxn
Full Name
Paxillin
Weight
64019 MW
Superfamily
paxillin family
Alternative Names
Paxillin;Pxn; PXN paxillin paxillin|testicular tissue protein Li 134
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pxn, check out the Pxn Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pxn: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) (MA1080)
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Customer Q&As
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16 Customer Q&As for Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10)
Question
I was wanting to use your anti-Paxillin antibody (Monoclonal, PXC-10) for WB for chicken cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for chicken cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It shows on the product datasheet, MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) has been validated for ICC, WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
I see that the anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The anti-Paxillin antibody (Monoclonal, PXC-10) (MA1080) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-30
Question
We have been able to see staining in chicken placenta. Any tips? Is anti-Paxillin antibody (Monoclonal, PXC-10) supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to literature placenta does express PXN. According to Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-12-06
Question
Our lab used your anti-Paxillin antibody (Monoclonal, PXC-10) for ICC on liver a few months ago. I am using rat, and We want to use the antibody for WB next. you antibody examining liver as well as fundus of stomach in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
I have checked the website and datasheets of our anti-Paxillin antibody (Monoclonal, PXC-10) and I see that MA1080 has been tested on rat in both ICC and WB. Thus MA1080 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-22
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Paxillin antibody (Monoclonal, PXC-10) MA1080. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
Is a blocking peptide available for product anti-Paxillin antibody (Monoclonal, PXC-10) (MA1080)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do provide the blocking peptide for product anti-Paxillin antibody (Monoclonal, PXC-10) (MA1080). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-14
Question
Does MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
It shows on the product datasheet, MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-09
Question
My question regarding product MA1080, anti-Paxillin antibody (Monoclonal, PXC-10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Yang
Verified customer
Asked: 2018-03-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1080 anti-Paxillin antibody (Monoclonal, PXC-10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-22
Question
We need to test anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 on chicken cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
The products we sell, including anti-Paxillin antibody (Monoclonal, PXC-10) MA1080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-17
Question
Does anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PXN is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-07-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Paxillin antibody (Monoclonal, PXC-10) MA1080. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-01
Question
My boss were satisfied with the WB result of your anti-Paxillin antibody (Monoclonal, PXC-10). However we have been able to see positive staining in brain testis cytoskeleton using this antibody. Is that expected? Could you tell me where is PXN supposed to be expressed?
F. Bhatt
Verified customer
Asked: 2017-04-18
Answer
Based on literature, brain testis does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2017-04-18
Question
Is this MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) reactive to the isotypes of PXN?
V. Wu
Verified customer
Asked: 2014-07-07
Answer
The immunogen of MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) is C-terminal part of recombinant chicken paxillin(amino acids 305-559). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-07
Question
Do you have a BSA free version of anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 available?
D. Bhatt
Verified customer
Asked: 2013-10-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Paxillin antibody (Monoclonal, PXC-10) MA1080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-10-22
Question
I am looking for using your anti-Paxillin antibody (Monoclonal, PXC-10) for growth hormone receptor signaling pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
A. Krishna
Verified customer
Asked: 2013-07-31
Answer
Thanks for your inquiry. This MA1080 anti-Paxillin antibody (Monoclonal, PXC-10) is validated on 293t cell lysate, hela cell lysate, mm231 cell lysate, jurkat cell lysate. It is guaranteed to work for ICC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-07-31
