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Product Info Summary
| SKU: | M00669 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PAX5/Bsap Rabbit Monoclonal Antibody
SKU/Catalog Number
M00669
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX5/Bsap Rabbit Monoclonal Antibody catalog # M00669. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX5/Bsap Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00669)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ICD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00669 is reactive to PAX5 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
42149 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00669 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:30
Positive Control
IHC: human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of PAX5 expression in Ramos cell lysate.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using PAX5 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Ramos cells, using PAX5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PAX5 (Source: Uniprot.org, NCBI)
Gene Name
PAX5
Full Name
Paired box protein Pax-5
Weight
42149 MW
Alternative Names
Paired box protein Pax-5;B-cell-specific transcription factor;BSAP;PAX5; PAX5 ALL3, BSAP paired box 5 paired box protein Pax-5|B-cell lineage specific activator|paired box homeotic gene 5|paired domain gene 5|transcription factor PAX 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX5, check out the PAX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX5/Bsap Rabbit Monoclonal Antibody (M00669)
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No publications found for M00669
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Customer Q&As
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4 Customer Q&As for Anti-PAX5/Bsap Rabbit Monoclonal Antibody
Question
My question regarding product M00669, anti-PAX5/Bsap Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00669 anti-PAX5/Bsap Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-15
Question
I see that the anti-PAX5/Bsap Rabbit Monoclonal antibody M00669 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-24
Question
I was wanting to use your anti-PAX5/Bsap Rabbit Monoclonal antibody for WB for mouse buccal mucosa cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse buccal mucosa cell identification?
R. Lewis
Verified customer
Asked: 2016-08-24
Answer
It shows on the product datasheet, M00669 anti-PAX5/Bsap Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-PAX5/Bsap Rabbit Monoclonal antibody M00669. Let me know if you need anything else.
B. Krishna
Verified customer
Asked: 2014-11-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-05
