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Product Info Summary
| SKU: | A05228 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Zinc finger protein Helios IKZF2 Antibody
SKU/Catalog Number
A05228
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein Helios IKZF2 Antibody (Catalog # A05228). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IKZF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Zinc finger protein Helios IKZF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05228)
Host
Rabbit
Contents
IKZF2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IKZF2 antibody was raised against a 19 amino acid peptide near the amino terminus of human IKZF2. The immunogen is located within amino acids 50 - 100 of IKZF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IKZF2 antibody is human, mouse and rat reactive. At least two isoforms of IKZF2 are known to exist; this antibody will detect both isoforms. IKZF2 antibody is predicted to not cross-react with other IKZF proteins.
Reactive Species
A05228 is reactive to IKZF2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
59574 MW
Background of Helios
The Ikaros family zinc finger 2 (IKZF2) is a member of the Ikaros family of zinc-finger proteins, a family of hematopoietic-specific transcription factors involved in the regulation of lymphocyte development (1,2). IKZF2 forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development (3). Its expression is a marker for T cell activation and proliferation (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05228 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IKZF2 antibody can be used for detection of IKZF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IKZF2 in EL4 cell lysate with IKZF2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of IKZF2 in human spleen tissue with IKZF2 antibody at 5 μg/mL.
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Immunofluorescence of IKZF2 in human spleen tissue with IKZF2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IKZF2 (Source: Uniprot.org, NCBI)
Gene Name
IKZF2
Full Name
Zinc finger protein Helios
Weight
59574 MW
Superfamily
Ikaros C2H2-type zinc-finger protein family
Alternative Names
Ikaros family zinc finger 2 (Helios), ANF1A2, HELIOS, ZNF1A2, ZNFN1A2 Ikzf2|A730095J18Rik, He, Helios, Zfpn, Zfpn1a2, Znfn1a2|IKAROS family zinc finger 2|zinc finger protein Helios|ikaros family zinc finger protein 2|zinc finger protein, subfamily 1A, 2 (Helios)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKZF2, check out the IKZF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKZF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zinc finger protein Helios IKZF2 Antibody (A05228)
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