Click image to see more details
Product Info Summary
| SKU: | PB9700 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PAR2/F2RL1 Antibody Picoband®
SKU/Catalog Number
PB9700
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAR2/F2RL1 Antibody Picoband® catalog # PB9700. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAR2/F2RL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9700)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PAR2, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9700 is reactive to F2RL1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
44126 MW
Background of PAR2
Protease activated receptor 2 (PAR2), also known as coagulation factor II (thrombin) receptor-like 1 (F2RL1) or G-protein coupled receptor 11 (GPR11), is a protein that in humans is encoded by the F2RL1 gene. F2RL1 is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. Additionally, PAR2 modulates inflammatory responses and acts as a sensor for proteolytic enzymes generated during infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9700 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, COLO320 Whole Cell, SW620 Whole Cell, HEPG2 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PAR2 using anti-PAR2 antibody (PB9700).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: COLO320 Whole Cell Lysate,
Lane 3: SW620 Whole Cell Lysate,
Lane 4: HEPG2 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # PB9700) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 49 kDa. The expected band size for PAR2 is at 44 kDa.
Protein Target Info & Infographic
Gene/Protein Information For F2RL1 (Source: Uniprot.org, NCBI)
Gene Name
F2RL1
Full Name
Proteinase-activated receptor 2
Weight
44126 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Proteinase-activated receptor 2;PAR-2;Coagulation factor II receptor-like 1;G-protein coupled receptor 11;Thrombin receptor-like 1;Proteinase-activated receptor 2, alternate cleaved 1;Proteinase-activated receptor 2, alternate cleaved 2;F2RL1;GPR11, PAR2; F2RL1 GPR11, PAR2 F2R like trypsin receptor 1 proteinase-activated receptor 2|G-protein coupled receptor 11|coagulation factor II (thrombin) receptor-like 1|protease-activated receptor 2|thrombin receptor-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F2RL1, check out the F2RL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F2RL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAR2/F2RL1 Antibody Picoband® (PB9700)
Hello CJ!
PB9700 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
D. QUANJUN, Z. QINGYU, Z. QILIANG, X. LIQUN, C. JINMEI, L. ZIQUAN, H. SHIKE Eur Rev Med Pharmacol Sci. 2016 Nov;20(22):4688-4696. Effect and mechanism of PAR-2 on the proliferation of esophageal cancer cells.
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PAR2/F2RL1 Antibody Picoband®
Question
Will anti-PAR2/F2RL1 antibody PB9700 work for WB with colonic mucosa?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of colonic mucosa, F2RL1 is highly expressed in colonic mucosa. So, it is likely that anti-PAR2/F2RL1 antibody PB9700 will work for WB with colonic mucosa.
Boster Scientific Support
Answered: 2020-03-31
Question
I see that the anti-PAR2/F2RL1 antibody PB9700 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colonic mucosa using anti-PAR2/F2RL1 antibody PB9700. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
Would PB9700 anti-PAR2/F2RL1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
As indicated on the product datasheet, PB9700 anti-PAR2/F2RL1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-26
Question
Do you have a BSA free version of anti-PAR2/F2RL1 antibody PB9700 available?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAR2/F2RL1 antibody PB9700 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-16
Question
We are currently using anti-PAR2/F2RL1 antibody PB9700 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
R. Collins
Verified customer
Asked: 2016-05-16
Answer
The anti-PAR2/F2RL1 antibody (PB9700) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-16
Question
Please see the WB image, lot number and protocol we used for colonic mucosa using anti-PAR2/F2RL1 antibody PB9700. Please let me know if you require anything else.
C. Johnson
Verified customer
Asked: 2013-08-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-08
