Product Info Summary
SKU: | A02205 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-DNA Polymerase thet POLQ Antibody
View all DNA polymerase theta Antibodies
SKU/Catalog Number
A02205
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA Polymerase thet POLQ Antibody (Catalog# A02205). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Polymerase thet POLQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02205)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POLQ. Immunogen sequence location: 181-230
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02205 is reactive to POLQ in Human, Mouse, Rat
Applications
A02205 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
289619 MW
Background of DNA polymerase theta
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DNA Polymerase thet Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLQ (Source: Uniprot.org, NCBI)
Gene Name
POLQ
Full Name
DNA polymerase theta
Weight
289619 MW
Superfamily
DNA polymerase type-A family
Alternative Names
DKFZp781A0112; DNA polymerase eta; EC 2.7.7.7; POLHDNA polymerase theta; polymerase (DNA directed), theta; PRO0327 POLQ PRO0327 DNA polymerase theta DNA polymerase theta|epididymis secretory sperm binding protein|polymerase (DNA directed), theta|polymerase (DNA) theta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLQ, check out the POLQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA Polymerase thet POLQ Antibody (A02205)
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No publications found for A02205
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-DNA Polymerase thet POLQ Antibody
Question
I see that the anti-DNA Polymerase thet antibody A02205 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-06
Question
Does anti-DNA Polymerase thet antibody A02205 work on canine IHC with secondary oocyte?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not tested anti-DNA Polymerase thet antibody A02205 on canine. You can run a BLAST between canine and the immunogen sequence of anti-DNA Polymerase thet antibody A02205 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine secondary oocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A02205 anti-DNA Polymerase thet antibody reactive to the isotypes of POLQ?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of A02205 anti-DNA Polymerase thet antibody is A peptide derived from human POLQ. Immunogen sequence location: 181-230. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
Does anti-DNA Polymerase thet antibody A02205 work for IHC with spleen?
A. Huang
Verified customer
Asked: 2019-03-25
Answer
According to the expression profile of spleen, POLQ is highly expressed in spleen. So, it is likely that anti-DNA Polymerase thet antibody A02205 will work for IHC with spleen.
Boster Scientific Support
Answered: 2019-03-25
Question
We are currently using anti-DNA Polymerase thet antibody A02205 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The anti-DNA Polymerase thet antibody (A02205) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-04
Question
I was wanting to use your anti-DNA Polymerase thet antibody for IHC for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
You can see on the product datasheet, A02205 anti-DNA Polymerase thet antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-14