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Product Info Summary
| SKU: | MA1082 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26)
SKU/Catalog Number
MA1082
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
PCK-26
Isotype
Mouse IgG1
Immunogen
Cytokeratin from human epidermis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1082 is reactive to Krt8 in Human, Mouse
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
54019 MW
Background of KRT8
Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1082 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunofluorescence, 1-2μg/ml, Human, -
Positive Control
WB: human NCL-H460 whole cell, human Hela whole cell, human A431 whole cell, mouse skin tissue
IHC: human lung cancer tissue
IF: human intestine cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human NCL-H460 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Pan cytokeratin antigen affinity purified monoclonal antibody (Catalog # MA1082) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Pan cytokeratin at approximately 54 kDa. The expected band size for Pan cytokeratin is at 54 kDa.
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Figure 2. IHC analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Pan cytokeratin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 3. IF analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Pan cytokeratin was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody at 1: 500 and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Krt8 (Source: Uniprot.org, NCBI)
Gene Name
Krt8
Full Name
Keratin, type II cytoskeletal 8
Weight
54019 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8; KRT8 CARD2, CK-8, CK8, CYK8, K2C8, K8, KO keratin 8 keratin, type II cytoskeletal 8|cytokeratin-8|keratin 8, type II|type-II keratin Kb8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Krt8, check out the Krt8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Krt8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) (MA1082)
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Customer Q&As
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3 Customer Q&As for Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26)
Question
Does anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 work on zebrafish WB with testis?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
Our lab technicians have not tested anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-01
Question
See below the WB image, lot number and protocol we used for colon using anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082. Please let me know if you require anything else.
P. Williams
Verified customer
Asked: 2015-12-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-29
Question
I see that the anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 works with WB, what is the protocol used to produce the result images on the product page?
J. Miller
Verified customer
Asked: 2013-05-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-20
