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- Table of Contents
Facts about Keratin, type II cytoskeletal 8.
Human | |
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Gene Name: | KRT8 |
Uniprot: | P05787 |
Entrez: | 3856 |
Belongs to: |
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intermediate filament family |
CARD2; CK8; CK-8; CYK8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8
Mass (kDA):
53.704 kDA
Human | |
---|---|
Location: | 12q13.13 |
Sequence: | 12; NC_000012.12 (52897191..52949860, complement) |
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
If you're interested in knowing more about Boster Bio and how it can be used in your lab, contact Sanbio. They can provide all the information you need and arrange for BeNeLux delivery. They also offer customized services to their customers. Sanbio can assist you in learning more about Boster Bio. It's worth spending a little time on their website, and we're certain you'll be glad you did!
If you're interested in qRT-PCR and want to know more about Boster Bio's qRT PCR kits be sure to check out the marker KRT8. This marker can be used to detect the presence of biomarkers in the development of cancers, developmental biology, inflammation, and other conditions. This marker has been tested on various samples and has produced reliable results. Boster Bio provides technical support 24 hours a day and provides technical support for its customers.
The fluorescent dye is released by markers attached to viral DNA segments. After each cycle, the machine tracks the fluorescence level in the sample. The virus is identified when the fluorescence level exceeds an amount. To determine the severity of infection, the number of cycles required to achieve this level is determined. It is possible for an individual sample to be identified by the KRT8 marker after more than one cycle.
The RT-qPCR array was designed to identify 41 genes that are involved in critical cellular pathways. It was developed using 100-well discs compatible to the Rotor-Gene instrument, however, it can be easily modified to plates that have 96 wells. The qRTPCR array was created using multiple technical controls to evaluate the final results. For example six wells were set aside for the absence of template controls and housekeeping genes, and one PCR was conducted in each well.
Boster Bio's qRT-PCR has tested for SARS and HIV in the presence of. It is highly sensitive and precise, as well as reliable. This product is designed to be used in laboratories that suspect the presence of the virus. The test results can be accessed in less than 30 minutes. The test is reliable and fast, which makes it a perfect choice to determine the extent of an infection.
The mRNAs used in qRT-PCR are derived from hepatocytes that were isolated from male C57BL/6 mice. Before transfection cells were fixed with formaldehyde 4 hours. The mRNPs were immunopurified with an anti-IGF2BP1 monoclone antibody. The cells were stained with a monoclone antibody against IGF2BP1 then analysed using qRT-PCR.
In an investigation of 451 clinical samples, the Bio-Speedy(r) Direct RT-qPCR kit used for SARS-CoV-2 utilized in conjunction with a Real-Time PCR kit that was approved by the Food and Drug Administration. Samples were obtained from people who were diagnosed with COVID-19 or the disease. There was no restriction on the age, gender or medications used by patients.
The Bio-Speedy(r), Direct RTPCR SARS CoV-2 kit requires that only authorized laboratories use it in accordance with the instructions. The labeling that is authorized also requires that the laboratory uses the kit and adhere to the procedures and control materials. The data generated from the study should be disclosed to the appropriate public health authorities. If the test results reveal that the KRT8 marker was present in an infected individual the bio-Speedy(r) Direct RT-qPCRSARS CoV-2 Kit should be reported to the appropriate authorities.
PMID: 1691124 by Krauss S., et al. Organization and sequence of the human gene encoding cytokeratin 8.
PMID: 1692965 by Yamamoto R., et al. Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP.
*More publications can be found for each product on its corresponding product page