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Product Info Summary
| SKU: | M01421-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01421-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody catalog # M01421-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01421-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CIG-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01421-2 is reactive to KRT8 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
53704 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01421-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: Mouse heart tissue, Rat skin tissue, Human liver tissue, human liver tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6W dilution for 1 hour at room temperature.
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Western blot analysis of Cytokeratin 8 expression in HaCaT cell lysate.
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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver, using the Antibody at 1:600 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For KRT8 (Source: Uniprot.org, NCBI)
Gene Name
KRT8
Full Name
Keratin, type II cytoskeletal 8
Weight
53704 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type II cytoskeletal 8;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;KRT8;CYK8; KRT8 CARD2, CK-8, CK8, CYK8, K2C8, K8, KO keratin 8 keratin, type II cytoskeletal 8|cytokeratin-8|keratin 8, type II|type-II keratin Kb8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT8, check out the KRT8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody (M01421-2)
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