Product Info Summary
SKU: | M02320 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD151 Purified Monoclonal Antibody
SKU/Catalog Number
M02320
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD151 Purified Monoclonal Antibody (Catalog# M02320). Tested in IHC-F, WB, Flow Cytometry, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD151 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02320)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
50-6
Isotype
Mouse IgG1 kappa
Immunogen
Human epidermoid carcinoma cell line Hep-3. The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02320 is reactive to CD151 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
28295 MW
Background of CD151
CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02320 is guaranteed for Flow Cytometry, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-4 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of CD151 using Anti-Hu CD151 Purified Monoclonal Antibody (M02320).
Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD151 (50-6) PE.Protein Target Info & Infographic
Gene/Protein Information For CD151 (Source: Uniprot.org, NCBI)
Gene Name
CD151
Full Name
CD151 antigen
Weight
28295 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
RAPH blood group, MER2, SFA1, PETA-3, GP27 CD151 GP27, MER2, PETA-3, RAPH, SFA1, TSPAN24 CD151 molecule (Raph blood group) CD151 |CD151 (Raph blood group)|hemidesmosomal tetraspanin CD151|membrane glycoprotein SFA-1|platelet surface glycoprotein gp27|platelet-endothelial cell tetraspan 3|platelet-endothelial tetraspan 3|tetraspanin-24|tspan-24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD151, check out the CD151 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD151: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD151 Purified Monoclonal Antibody (M02320)
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