Anti-p73/TP73 Antibody Picoband®

Product Name

Anti-p73/TP73 Antibody Picoband®

View all p73 Antibodies

SKU/Catalog Number

PB9235

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p73/TP73 Antibody Picoband® catalog # PB9235. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p73/TP73 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9235)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human p73 recombinant protein (Position: M1-E198). Human p73 shares 96% amino acid (aa) sequence identity with mouse p73.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9235 is reactive to TP73 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

73 kDa

Calculated molecular weight

69623 MW

Background of p73

p73, also known as tumor protein 73 (TP73), encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It is mapped to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Furthermore, recent finding are suggesting that over-expression of transcription factors involved in cell cycle regulation and synthesis of DNA in mammalian cells (e.g.: E2F-1) induces the expression of p73. In addition, p73 is a substrate of the c-Abl kinase and that the ability of c-Abl to phosphorylate p73 is markedly increased by gamma-irradiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9235 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Whole Cell, MCF-7 Whole Cell, COLO320 Whole Cell
IHC: Human Intestinal Cancer Tissue, Rat Brain Tissue, Mouse Intestine Tissue

Validation Images & Assay Conditions

Gene/Protein Information For TP73 (Source: Uniprot.org, NCBI)

Alternative Names

Tumor protein p73;p53-like transcription factor;p53-related protein;TP73;P73; TP73 P73 tumor protein p73 tumor protein p73|p53-like transcription factor|p53-related protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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