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Product Info Summary
| SKU: | M03959-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p60 CAF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03959-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-p60 CAF1 Rabbit Monoclonal Antibody catalog # M03959-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p60 CAF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03959-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20C00
Isotype
IgG
Immunogen
A synthesized peptide derived from human p60 CAF1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03959-1 is reactive to CHAF1B in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
55990 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03959-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: human CACO-2 whole cell, human SiHa whole cell, human HEL whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p60 CAF1 using anti-p60 CAF1 antibody (M03959-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p60 CAF1 antigen affinity purified monoclonal antibody (Catalog # M03959-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p60 CAF1 at approximately 70 kDa. The expected band size for p60 CAF1 is at 61 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CHAF1B (Source: Uniprot.org, NCBI)
Gene Name
CHAF1B
Full Name
Chromatin assembly factor 1 subunit B
Weight
55990 MW
Superfamily
WD repeat HIR1 family
Alternative Names
Perilipin-1;Lipid droplet-associated protein;PLIN1;PERI, PLIN; CHAF1B CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 chromatin assembly factor 1 subunit B chromatin assembly factor 1 subunit B|CAF-1 subunit B|CAF-I 60 kDa subunit|CAF-I p60|M-phase phosphoprotein 7|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHAF1B, check out the CHAF1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHAF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p60 CAF1 Rabbit Monoclonal Antibody (M03959-1)
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