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Product Info Summary
| SKU: | A30479 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CAF1B CHAF1B Antibody
SKU/Catalog Number
A30479
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CAF1B CHAF1B Antibody (Catalog# A30479). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAF1B CHAF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30479)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CAF1B. Immunogen sequence location: 71-120
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30479 is reactive to CHAF1B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
61493 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30479 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CHAF1B (Source: Uniprot.org, NCBI)
Gene Name
CHAF1B
Full Name
Chromatin assembly factor 1 subunit B
Weight
61493 MW
Superfamily
WD repeat HIR1 family
Alternative Names
CHAF1B;CAF1A;CAF1P60;MPHOSPH7;MPP7;Chromatin assembly factor 1 subunit B;CAF-1 subunit B;Chromatin assembly factor I p60 subunit;CAF-I 60 kDa subunit;CAF-I p60;M-phase phosphoprotein 7 CHAF1B CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 chromatin assembly factor 1 subunit B chromatin assembly factor 1 subunit B|CAF-1 subunit B|CAF-I 60 kDa subunit|CAF-I p60|M-phase phosphoprotein 7|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHAF1B, check out the CHAF1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHAF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAF1B CHAF1B Antibody (A30479)
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No publications found for A30479
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CAF1B CHAF1B Antibody
Question
Would anti-CAF1B antibody A30479 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CHAF1B is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-CAF1B antibody A30479 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-03-31
Question
Would A30479 anti-CAF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Collins
Verified customer
Asked: 2020-03-10
Answer
As indicated on the product datasheet, A30479 anti-CAF1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-10
Question
Do you have a BSA free version of anti-CAF1B antibody A30479 available?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CAF1B antibody A30479 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-11
Question
I see that the anti-CAF1B antibody A30479 works with WB, what is the protocol used to produce the result images on the product page?
M. Jones
Verified customer
Asked: 2019-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-CAF1B antibody A30479 work on pig WB with cervix carcinoma erythroleukemia?
B. Li
Verified customer
Asked: 2018-10-24
Answer
Our lab technicians have not validated anti-CAF1B antibody A30479 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CAF1B antibody A30479 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-24
Question
We are currently using anti-CAF1B antibody A30479 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
S. Edwards
Verified customer
Asked: 2018-03-02
Answer
The anti-CAF1B antibody (A30479) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-02
