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Product Info Summary
| SKU: | M00176-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245
SKU/Catalog Number
M00176-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245 (Catalog # M00176-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00176-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM245
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to human p38 MAPK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human p38 MAPK. This antibody may also react to rat and mouse p38 MAPK, as predicted by immunogen homology.
Reactive Species
M00176-3 is reactive to MAPK14 in Human
Reconstitution
Calculated molecular weight
41293 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00176-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:5000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cells lysates using Anti-p38 MAPK RM245 at a 1:1000 dilution, showed a band of p38 MAPK (~38 kDa) expressed in HeLa cells.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-p38 MAPK RM245 at a 1:5000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MAPK14 (Source: Uniprot.org, NCBI)
Gene Name
MAPK14
Full Name
Mitogen-activated protein kinase 14
Weight
41293 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;2.7.11.24 ;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein 2;Mitogen-activated protein kinase p38 alpha;MAP kinase p38 alpha;Stress-activated protein kinase 2a;SAPK2a;MAPK14;CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A; MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK14, check out the MAPK14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245 (M00176-3)
Hello CJ!
M00176-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245
Question
Is this M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 reactive to the isotypes of MAPK14?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The immunogen of M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 is A peptide corresponding to human p38 MAPK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-29
Question
Is there a BSA free version of anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 available?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-02
Question
Is a blocking peptide available for product anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 (M00176-3)?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
We do provide the blocking peptide for product anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 (M00176-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
We have seen staining in human platelet. Do you have any suggestions? Is anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
From literature platelet does express MAPK14. From Uniprot.org, MAPK14 is expressed in blood, peripheral blood, liver, b-cell, renal cell carcinoma, placenta, platelet, hepatoma, embryonic kidney, cervix carcinoma, leukemic t-cell, t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2019-10-18
Question
We are interested in to test anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 on human t-cell for research purposes, then I may be interested in using anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The products we sell, including anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use your anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 for IHC for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
N. Brown
Verified customer
Asked: 2019-05-24
Answer
The anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 (M00176-3) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-10
Question
Will M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
It shows on the product datasheet, M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-18
Question
Our lab used your anti-p38 MAPK Rabbit Monoclonal antibody, Clone
T. Miller
Verified customer
Asked: 2018-11-12
Answer
I took a look at the website and datasheets of our anti-p38 MAPK Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-11-12
Question
My colleagues were well pleased with the WB result of your anti-p38 MAPK Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
According to literature, embryonic kidney does express MAPK14. Generally MAPK14 expresses in cytoplasm. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2018-10-16
Question
Our lab want to know about using your anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 for lipopolysaccharide-mediated signaling pathway studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
We appreciate your inquiry. This M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 is validated on hela cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-15
Question
I have a question about product M00176-3, anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00176-3 anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-23
Question
See below the WB image, lot number and protocol we used for t-cell using anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-06
Question
Does anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 work for IHC with t-cell?
M. Huang
Verified customer
Asked: 2016-06-29
Answer
According to the expression profile of t-cell, MAPK14 is highly expressed in t-cell. So, it is likely that anti-p38 MAPK Rabbit Monoclonal antibody, Clone#RM245 M00176-3 will work for IHC with t-cell.
Boster Scientific Support
Answered: 2016-06-29
