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Product Info Summary
| SKU: | M04136-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1)
View all p23/PTGES3 Antibodies
SKU/Catalog Number
M04136-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) catalog # M04136-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04136-3)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
9D3D1
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human p23/PTGES3 recombinant protein (Position: M1-K79).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M04136-3 is reactive to PTGES3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
18.697kDa
Background of p23/PTGES3
Prostaglandin E synthase 3 (cytosolic)is anenzymethat in humans is encoded by the PTGES3gene. It is mapped to 12q13.3; 12. This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04136-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human COLO-320 whole cell, human K562 whole cell, rat liver tissue, rat RH35 whole cell, mouse HEPA1-6 whole cell
ICC/IF: T-47D cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p23/PTGES3 antigen affinity purified monoclonal antibody (Catalog # M04136-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p23/PTGES3 at approximately 23 kDa. The expected band size for p23/PTGES3 is at 23 kDa.
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Figure 2. IF analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3).
p23/PTGES3 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-p23/PTGES3 Antibody (M04136-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-p23/PTGES3 antibody (M04136-3).
Overlay histogram showing PC-3 cells stained with M04136-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-p23/PTGES3 Antibody (M04136-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PTGES3 (Source: Uniprot.org, NCBI)
Gene Name
PTGES3
Full Name
Prostaglandin E synthase 3
Weight
18.697kDa
Superfamily
p23/wos2 family
Alternative Names
SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2, UBLE1B; HRIHFB2115 PTGES3 P23, TEBP, cPGES prostaglandin E synthase 3 prostaglandin E synthase 3|Hsp90 co-chaperone|cytosolic prostaglandin E synthase|cytosolic prostaglandin E2 synthase|progesterone receptor complex p23|prostaglandin E synthase 3 (cytosolic)|telomerase-binding protein p23|unactive progesterone receptor, 23 kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGES3, check out the PTGES3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGES3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) (M04136-3)
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