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Product Info Summary
| SKU: | M00145-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
View all p21/CIP1/CDKN1A Antibodies
SKU/Catalog Number
M00145-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-3. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p21 CDKN1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p21
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00145-3 is reactive to CDKN1A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
18119 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00145-3 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of MCF7 cells, using p21 Antibody .
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Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) LnCaP cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDKN1A (Source: Uniprot.org, NCBI)
Gene Name
CDKN1A
Full Name
Cyclin-dependent kinase inhibitor 1
Weight
18119 MW
Superfamily
CDI family
Alternative Names
Cyclin-dependent kinase inhibitor 1;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;CDKN1A;CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1; CDKN1A CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 cyclin dependent kinase inhibitor 1A cyclin-dependent kinase inhibitor 1|CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1A (p21, Cip1)|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1A, check out the CDKN1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p21 CDKN1A Rabbit Monoclonal Antibody (M00145-3)
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Customer Q&As
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1 Customer Q&As for Anti-p21 CDKN1A Rabbit Monoclonal Antibody
Question
We are currently using anti-p21 Rabbit Monoclonal antibody M00145-3 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The anti-p21 Rabbit Monoclonal antibody (M00145-3) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-24
