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Product Info Summary
| SKU: | M00145-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
View all p21/CIP1/CDKN1A Antibodies
SKU/Catalog Number
M00145-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-2. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p21 CDKN1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EAI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p21
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00145-2 is reactive to CDKN1A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
18119 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00145-2 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: MCF-7 cell, HeLa cell
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) HeLa cell lysate. (3) LnCap cell lysate; (4) U87 MG cell lysate.
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Immunofluorescent analysis of MCF7 cells, using p21 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDKN1A (Source: Uniprot.org, NCBI)
Gene Name
CDKN1A
Full Name
Cyclin-dependent kinase inhibitor 1
Weight
18119 MW
Superfamily
CDI family
Alternative Names
Cyclin-dependent kinase inhibitor 1;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;CDKN1A;CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1; CDKN1A CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 cyclin dependent kinase inhibitor 1A cyclin-dependent kinase inhibitor 1|CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1A (p21, Cip1)|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1A, check out the CDKN1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p21 CDKN1A Rabbit Monoclonal Antibody (M00145-2)
Hello CJ!
M00145-2 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen Q,Fu L,Hu J,Guo G,Xie A.Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration.Cancer Cell Int.2021 Apr 26;21(1):235.doi:10.1186/s12935-021-01934-8.PMID:33902600;PMCID:PMC8077794.
Species: Human,Mouse
M00145-2 usage in article: APP:IHC, SAMPLE:PROSTATE CANCER TISSUE, DILUTION:NA
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
M00145-2 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Yi L,Ju Y,He Y,Yin X,Xu Y,Weng T.Intraperitoneal injection of Desferal® alleviated the age-related bone loss and senescence of bone marrow stromal cells in rats.Stem Cell Res Ther.2021 Jan 7;12(1):45.doi:10.1186/s13287-020-02112-9.PMID:33413663;PMCID:PMC7
Species: Rat
M00145-2 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
Minicircle-oriP-IFN?: A Novel Targeted Gene Therapeutic System for EBV Positive Human Nasopharyngeal Carcinoma
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Teng-Long-Bu-Zhong-Tang, a Chinese herbal formula, enhances anticancer effects of 5 - Fluorouracil in CT26 colon carcinoma
Action Mechanism of Inhibin ?-Subunit on the Development of Sertoli Cells and First Wave of Spermatogenesis in Mice
Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...
Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal C...
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