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Product Info Summary
| SKU: | A03299 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p18 INK CDKN2C Antibody
View all p18INK4c/CDKN2C Antibodies
SKU/Catalog Number
A03299
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-p18 INK CDKN2C Antibody (Catalog # A03299). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p18 INK CDKN2C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03299)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p18 INK antibody.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03299 is reactive to CDKN2C in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
18127 MW
Background of p18INK4c/CDKN2C
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Youzhong Yuan, Cancer Res., Jan 2006; 66: 343 - 351.
A. Koenig, Vet. Pathol., Jul 2002; 39: 458.
Pei-Fang Hung, Am J Physiol Cell Physiol, May 2005; 288: C1094 - C1108.
Mana Mirjany, J. Pharmacol. Exp. Ther., May 2002; 301: 494.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03299 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from COS7 cells, using p18 INK antibody A03299.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p18 INK antibody A03299.
Protein Target Info & Infographic
Gene/Protein Information For CDKN2C (Source: Uniprot.org, NCBI)
Gene Name
CDKN2C
Full Name
Cyclin-dependent kinase 4 inhibitor C
Weight
18127 MW
Superfamily
CDKN2 cyclin-dependent kinase inhibitor family
Alternative Names
Cyclin-dependent kinase 4 inhibitor C;Cyclin-dependent kinase 6 inhibitor;p18-INK4c;p18-INK6;CDKN2C;CDKN6; CDKN2C INK4C, p18, p18-INK4C cyclin dependent kinase inhibitor 2C cyclin-dependent kinase 4 inhibitor C|CDK6 inhibitor p18|cyclin-dependent inhibitor|cyclin-dependent kinase 6 inhibitor p18|cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)|p18-INK6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN2C, check out the CDKN2C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p18 INK CDKN2C Antibody (A03299)
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No publications found for A03299
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-p18 INK CDKN2C Antibody
Question
Would anti-p18 INK antibody A03299 work for IHC with adipose tissue?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of adipose tissue, CDKN2C is highly expressed in adipose tissue. So, it is likely that anti-p18 INK antibody A03299 will work for IHC with adipose tissue.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-p18 INK antibody (A03299)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do provide the blocking peptide for product anti-p18 INK antibody (A03299). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-03
Question
Do you have a BSA free version of anti-p18 INK antibody A03299 available?
R. Patel
Verified customer
Asked: 2018-04-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p18 INK antibody A03299 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-p18 INK antibody A03299. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-21
Question
We are currently using anti-p18 INK antibody A03299 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
The anti-p18 INK antibody (A03299) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-12
Question
Can you help my question with product A03299, anti-p18 INK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Roberts
Verified customer
Asked: 2015-09-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03299 anti-p18 INK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-22
