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Product Info Summary
| SKU: | M00016 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p16 INK Monoclonal Antibody
SKU/Catalog Number
M00016
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p16 INK Monoclonal Antibody catalog # M00016. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p16 INK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00016)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CGD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p16 INK
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00016 is reactive to CDKN2A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00016 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:100
Positive Control
IHC: human gastric tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of p16 INK expression in 293T cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human gastric, using p16 INK Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
Protein Target Info & Infographic
Gene/Protein Information For CDKN2A (Source: Uniprot.org, NCBI)
Gene Name
CDKN2A
Full Name
Cyclin-dependent kinase inhibitor 2A
Weight
88068 MW
Superfamily
CDKN2 cyclin-dependent kinase inhibitor family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN2A, check out the CDKN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p16 INK Monoclonal Antibody (M00016)
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No publications found for M00016
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Customer Q&As
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12 Customer Q&As for Anti-p16 INK Monoclonal Antibody
Question
Is this M00016 anti-p16 INK Monoclonal antibody reactive to the isotypes of CDKN2A?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The immunogen of M00016 anti-p16 INK Monoclonal antibody is A synthesized peptide derived from human p16 INK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-05
Question
Here is the WB image, lot number and protocol we used for adenohypophysis using anti-p16 INK Monoclonal antibody M00016. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16 INK Monoclonal antibody M00016. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
We are interested in to test anti-p16 INK Monoclonal antibody M00016 on human adenohypophysis for research purposes, then I may be interested in using anti-p16 INK Monoclonal antibody M00016 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Huang
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-p16 INK Monoclonal antibody M00016, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-p16 INK Monoclonal antibody M00016 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-p16 INK Monoclonal antibody (M00016) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Would M00016 anti-p16 INK Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
As indicated on the product datasheet, M00016 anti-p16 INK Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-16
Question
Would anti-p16 INK Monoclonal antibody M00016 work for WB with adenohypophysis?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16 INK Monoclonal antibody M00016 will work for WB with adenohypophysis.
Boster Scientific Support
Answered: 2019-03-08
Question
Is a blocking peptide available for product anti-p16 INK Monoclonal antibody (M00016)?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
We do provide the blocking peptide for product anti-p16 INK Monoclonal antibody (M00016). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-20
Question
I see that the anti-p16 INK Monoclonal antibody M00016 works with WB, what is the protocol used to produce the result images on the product page?
E. Gonzalez
Verified customer
Asked: 2017-05-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-30
Question
I was wanting to use your anti-p16 INK Monoclonal antibody for WB for human adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adenohypophysis identification?
D. Edwards
Verified customer
Asked: 2014-01-29
Answer
It shows on the product datasheet, M00016 anti-p16 INK Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-29
Question
Is there a BSA free version of anti-p16 INK Monoclonal antibody M00016 available?
R. Krishna
Verified customer
Asked: 2013-11-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16 INK Monoclonal antibody M00016 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-11
Question
Can you help my question with product M00016, anti-p16 INK Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Carter
Verified customer
Asked: 2013-01-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00016 anti-p16 INK Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-29
