Product Info Summary
SKU: | PA1869 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BCRP/ABCG2 Antibody
View all ABCG2/CD338 Antibodies
SKU/Catalog Number
PA1869
BA3849-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1869. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCRP/ABCG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1869)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human BCRP/ABCG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1869 is reactive to ABCG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
72314 MW
Background of ABCG2/CD338
ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded that ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1869 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (PA1869).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCRP/ABCG2 antigen affinity purified polyclonal antibody (Catalog # PA1869) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCRP/ABCG2 at approximately 75 kDa. The expected band size for BCRP/ABCG2 is at 72 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABCG2 (Source: Uniprot.org, NCBI)
Gene Name
ABCG2
Full Name
Broad substrate specificity ATP-binding cassette transporter ABCG2
Weight
72314 MW
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family G member 2;Breast cancer resistance protein;CDw338;Mitoxantrone resistance-associated protein;Placenta-specific ATP-binding cassette transporter;Urate exporter;CD338;ABCG2;ABCP, BCRP, BCRP1, MXR; ABCG2 ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1 ATP binding cassette subfamily G member 2 (Junior blood group) broad substrate specificity ATP-binding cassette transporter ABCG2|ABC transporter|ATP-binding cassette transporter G2|ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)|breast cancer resistance protein|mitoxantrone resistance-associated protein|multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2|placenta specific MDR protein|placenta-specific ATP-binding cassette transporter|urate exporter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG2, check out the ABCG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCRP/ABCG2 Antibody (PA1869)
Hello CJ!
PA1869 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells
Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.
Qiao B, Johnson Nw, Chen X, Li R, Tao Q, Gao J. Oncol Rep. 2011 Aug;26(2):455-61. Doi: 10.3892/Or.2011.1299. Epub 2011 May 10. Disclosure Of A Stem Cell Phenotype In An Oral Squamous Cell Carcinoma Cell Line Induced By Bmp-4 Via An Epithelial-Mese...
Duan J, Mansour Hm, Zhang Y, Deng X, Chen Y, Wang J, Pan Y, Zhao J. Int J Pharm. 2012 Apr 15;426(1-2):193-201. Doi: 10.1016/J.Ijpharm.2012.01.020. Epub 2012 Jan 17. Reversion Of Multidrug Resistance By Co-Encapsulation Of Doxorubicin And Curcumin ...
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
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