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Product Info Summary
| SKU: | A01000-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MBL2 Antibody Picoband®
SKU/Catalog Number
A01000-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MBL2 Antibody Picoband® catalog # A01000-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01000-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse MBL2 recombinant protein (Position: L39-D229).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01000-3 is reactive to Mbl2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
26.144kDa
Background of MBL2
MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01000-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
ELISA (Cap),1-5μg/ml
Positive Control
WB: mouse liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
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Figure 2. Sandwich ELISA - Recombinant mouse MBL2 protein standard curve.
Use in combination with reagents from Mouse MBL2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0806).
Protein Target Info & Infographic
Gene/Protein Information For Mbl2 (Source: Uniprot.org, NCBI)
Gene Name
Mbl2
Full Name
Mannose-binding protein C
Weight
26.144kDa
Alternative Names
Mannose-binding protein C; MBP-C; Mannan-binding protein; RA-reactive factor P28A subunit; RARF/P28A; Mbl2 MBL2 COLEC1, HSMBPC, MBLD, MBP, MBP-C, MBP1, MBPD, MBL2 mannose binding lectin 2 mannose-binding protein C|collectin-1|mannan-binding lectin|mannose-binding lectin (protein C) 2, soluble (opsonic defect)|mannose-binding lectin 2, soluble (opsonic defect)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mbl2, check out the Mbl2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mbl2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBL2 Antibody Picoband® (A01000-3)
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Customer Q&As
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3 Customer Q&As for Anti-MBL2 Antibody Picoband®
Question
Does anti-MBL2 antibody A01000-3 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Our lab technicians have not tested anti-MBL2 antibody A01000-3 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MBL2 antibody A01000-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
I have a question about product A01000-3, anti-MBL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Taylor
Verified customer
Asked: 2017-07-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01000-3 anti-MBL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-25
Question
We are currently using anti-MBL2 antibody A01000-3 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
J. Parker
Verified customer
Asked: 2015-03-12
Answer
The anti-MBL2 antibody (A01000-3) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-12
