Key=store_id Key=entity_id, value=125611 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00804-3 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2024-01-25 08:17:13 Key=updated_at, value=2024-10-25 05:04:53 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Osm Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1207 Key=yoast_content_score, value=60 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Osm Antibody Key=meta_description, value=Boster Bio Anti-Osm Antibody Picoband® catalog # A00804-3. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. Key=image, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=small_image, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=thumbnail, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=url_key, value=anti-osm-picoband-trade-antibody-a00804-3-boster Key=url_path, value=anti-s6-kinase-1-thr449-antibody-phospho-specific-p30408-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of Osm using anti-Osm antibody (A00804-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample Key=small_image_label, value=Figure 1. Western blot analysis of Osm using anti-Osm antibody (A00804-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample Key=thumbnail_label, value=Figure 1. Western blot analysis of Osm using anti-Osm antibody (A00804-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100 µg/vial Key=protein_name, value=Serine/threonine-protein kinase AtPK1/AtPK6 Key=gene_full_name, value=Serine/threonine-protein kinase AtPK1/AtPK6 Key=uniprot_id, value=Q65Z15 Key=reconstitution, value=Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. Key=applications, value=ELISA, WB Key=reactivity, value=Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. Key=cross_reactivity, value=No cross reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=clone_number Key=predicted_reactivity Key=source_company, value=Boster: A00804-3 Key=isotype, value=IgG Key=molecular_weight, value=52588 MW Key=sample_size_available, value=25ug for $99, contact us for details Key=related_product_skus, value=PROTP19105 Key=product_category, value=Primary Antibodies Key=research_category, value=Signal Transduction, Signaling Pathway, Small G Proteins Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=rating_value, value=95 Key=review_count, value=1 Key=phospho_site Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/a/0/a00804-3-osm-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Colonic Neoplasms:P00151|EKC2549|A00151Y397-1^Senescence:PA2175|PB9121|M00229 Key=description, value=Boster Bio Anti-Osm Antibody Picoband® catalog # A00804-3. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-Osm Antibody Picoband® catalog # A00804-3. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-Osm Antibody Picoband® Key=contents, value=Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. Key=background, value=OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Key=gene_name, value=Osm Key=application_details, value=Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Key=subcellular_localization, value= Cytoplasm. Nucleus. Key=tissue_specificity, value= Expressed in all tissues. Key=protein_function, value= Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway. Key=application_notes, value=Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Key=competitor_equivalent_skus, value=sc 87080|sc 390685 Key=immunogen, value=E.coli-derived rat Osm recombinant protein (Position: E55-D158). Rat Osm shares 46% and 71.2% amino acid (aa) sequence identity with human and mouse Osm, respectively. Key=purification, value=Immunogen affinity purified. Key=storage, value=At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing. Key=custom_attribute_1, value=27 kDa Key=custom_attribute_2, value=WB: rat testis tissue, rat kidney tissue, rat C6 whole cell, mouse tetis tissue, mouse kidney tissue, mouse lung tissue, mouse RAW2647 whole cell Key=synonyms, value=70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Osm Antibody Picoband®
Oncostatin M/OSM antibody
Boster Bio Anti-Osm Antibody Picoband® catalog # A00804-3. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-Osm Antibody Picoband® catalog # A00804-3. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Cite This Product
Anti-Osm Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00804-3)
E.coli-derived rat Osm recombinant protein (Position: E55-D158). Rat Osm shares 46% and 71.2% amino acid (aa) sequence identity with human and mouse Osm, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00804-3 is reactive to Osm in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
52588 MW
Background of Oncostatin M/OSM
OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Positive Control
WB: rat testis tissue, rat kidney tissue, rat C6 whole cell, mouse tetis tissue, mouse kidney tissue, mouse lung tissue, mouse RAW2647 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Osm using anti-Osm antibody (A00804-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse tetis tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osm antigen affinity purified polyclonal antibody (Catalog # A00804-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osm at approximately 27 kDa. The expected band size for Osm is at 27 kDa.
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Osm, check out the Osm Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Osm: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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