Anti-Ornithine Decarboxylase-1 (ODC-1) Monoclonal Antibody

Product Name

Anti-Ornithine Decarboxylase-1 (ODC-1) Monoclonal Antibody

View all Ornithine Decarboxylase Antibodies

SKU/Catalog Number

M03138

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Ornithine Decarboxylase-1 (ODC-1) Monoclonal Antibody (Catalog # M03138). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Ornithine Decarboxylase-1 (ODC-1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03138)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ODC1/487

Isotype

IgG2a, kappa

Immunogen

Recombinant human ODC-1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03138 is reactive to ODC1 in Human, Mouse, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

51148 MW

Background of Ornithine Decarboxylase

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03138 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ODC1 (Source: Uniprot.org, NCBI)

Alternative Names

Ornithine decarboxylase;ODC;4.1.1.17;ODC1; ODC1 BABS, NEDBA, NEDBIA, ODC ornithine decarboxylase 1 ornithine decarboxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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