Product Info Summary
SKU: | PB9317 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Oct-1/POU2F1 Antibody Picoband™
SKU/Catalog Number
PB9317
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Oct-1/POU2F1 Antibody Picoband™ catalog # PB9317. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oct-1/POU2F1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9317)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9317 is reactive to POU2F1 in Human
Applications
PB9317 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
76472 MW
Background of 44105
POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Oct-1 Picoband antibody, PB9317, Western blotting
All lanes: Anti Oct-1 (PB9317) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
Click image to see more details
Anti-Oct-1 Picoband antibody, PB9317, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For POU2F1 (Source: Uniprot.org, NCBI)
Gene Name
POU2F1
Full Name
POU domain, class 2, transcription factor 1
Weight
76472 MW
Superfamily
POU transcription factor family
Alternative Names
FLJ42836; NF-A1; Oct-1; OCT1class 2, transcription factor 1; OTF1; POU class 2 homeobox 1; POU domain class 2, transcription factor 1; POU2F1 POU2F1 OCT1, OTF1, oct-1B POU class 2 homeobox 1 POU domain, class 2, transcription factor 1|NF-A1|OTF-1|Octamer-binding transcription factor-1|oct-1|octamer-binding protein 1|octamer-binding transcription factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POU2F1, check out the POU2F1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POU2F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Oct-1/POU2F1 Antibody Picoband™ (PB9317)
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Customer Q&As
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1 Customer Q&As for Anti-Oct-1/POU2F1 Antibody Picoband™
Question
We are currently using anti-Oct-1/POU2F1 antibody PB9317 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The anti-Oct-1/POU2F1 antibody (PB9317) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-05